健脾消癥方对人胃癌细胞MGC803凋亡和细胞周期的影响

目的:观察健脾消癥方对胃癌细胞株MGC803增殖、凋亡和细胞周期的影响。方法:(1)采用MTT法观察不同浓度的健脾消癥方对胃癌细胞株MGC803增殖的抑制作用;(2)使用Annexin V/PI荧光双染法检测健脾消癥方诱导肿瘤细胞进入凋亡的比例;(3)应用流式细胞仪检测健脾消癥方对MGC803细胞周期的影响。结果:(1)健脾消癥方对MGC803细胞有较强的抑制增殖的作用,并呈一定的浓度依赖性。(2)健脾消癥方高、中、低浓度组对胃癌细胞株MGC803作用24h后,早期凋亡率分别为49.83%、55.29%、16.73%。健脾消癥方高、中、低浓度组对胃癌细胞株MGC803作用48h后,早期凋亡率分别为84.52%、55.58%、51.76%。(3)与阴性对照组相比,健脾消癥方各浓度组对胃癌细胞株MGC803作用48h后,可将细胞阻滞于G2-M期,健脾消癥方高浓度组在G0-G1期前出现较明显的亚二倍体峰。结论:(1)健脾消癥方可明显抑制胃癌细胞株MGC803的增殖作用。(2)健脾消癥方对人胃癌细胞株MGC803有明显的诱导凋亡及阻滞细胞周期在G2-M期的作用。

Influence of Jianpi Xiaozheng Formula on Apoptosis and Cell Cycle of Gastric Carcinoma Cell line MGC803

Objective:To investigate the effect of Jianpi Xiaozheng formula on proliferation,apoptosis and cell cycle of gastric carcinoma cell line MGC803 in vitro.Methods:After gastric carcinoma cell line MGC803 was treated with Jianpi Xiaozheng Formula in different concentrations,cell proliferation was assessed using MTT assay.The apoptosis rate was examined by flow cytometer using Annexin V-FITC/PI double staining.Apoptosis and cell cycle were examined by flow cytometry(FCM).Results:Jianpi Xiaozheng Formula significantly inhibited the proliferation of MGC803 cells in a concentration-dependent manner.The apoptosis rate of high-concentration group,moderate-concentration group and low-concentration group in 24h were 49.83%,55.29%,16.73% and in 48h were 84.52%,55.58%,51.76%,respectively.As compared with the negative control group,when the cells were treated by high-concentration group,the percentage of the cells in G2-M phase increased in treatment group,which showed the cells were arrested at the G2-M phase of the cell cycle with the increased rate of cell apoptosis.Conclusion:Jianpi Xiaozheng Formula can inhabit the proliferation of MGC803,and can significantly induce apoptosis and arrest the cells at the G2-M phase of the cell cycle.