湿阻中焦证模型大鼠胃肠水通道蛋白3(AQP3)的病理特征性表达分布谱以及平胃散干预的研究

目的:研究湿阻中焦证动物模型胃肠组织AQP3的分布和含量以及平胃散的干预,揭示湿阻中焦证动物模型水通道蛋白病理特征性表达分布谱。方法:SD大鼠60只,雌雄各半,随机分空白组、空白给药组、模型组、平胃散组和自然恢复组,每组12只。用免疫组化技术测定胃肠组织AQP3的分布,用酶联免疫法(ELISA)测定胃肠组织AQP3的含量。结果:模型组AQP3分布在胃贲门和小肠中段的黏膜增加,在小肠中段的外膜增加。经平胃散干预后,AQP3分布在胃贲门的黏膜减少,与自然恢复组比较,在小肠中段的黏膜有所增加。结论:AQP3在湿阻中焦证胃肠特征性表达分布谱可能是湿阻中焦证的分子基础之一。平胃散对湿阻中焦证胃肠AQP3表达的增强可能是平胃散治疗湿阻中焦证的分子机理之一。

Study on Expressional Distribution Spectrum of the Pathological Features of Aquaporin(AQP3)in Animal Model on the Syndrome of Dampness Incumbering Middle Energizer in Gastrointestinal Tissue and the Intervention by Pingwei Powder

Objective:Based on the animal model of the syndrome of dampness incumbering middle energizer,study on the distribution and content of the aquaporin 3(AQP3)in the model was made to reveal its expressional distribution spectrum of the pathological features of aquaporin.Methods:There were 60 SD rats.Half of them were male.These rats were divided into blank group,blank group with drugs,model group,Pingwei Powder group and natural recovery group randomly,each group had 12 rats.The model of the syndrome of dampness incumbering middle energizer was established.The distribution of the AQP3 was measured by immunohistochemistry and the content of the AQP3 by enzyme-linked immuno sorbent assay(ELISA)in the gastrointestional tissue.Results:In the model of the syndrome of dampness incumbering middle energizer,expressional content of AQP3 increased in mucosa of cardiac stomach and middle part of small intestine,and also increased in adventitia of middle part of small intestine.After the intervention of Pingwei Power,expressional content of AQP3 reduced in mucosa of cardiac stomach.Comparing with the natural recovery group,the content of AQP3 increased in mucosa of middle part of small intestine.Conclusion:Expressional distribution spectrum of the pathological features of AQP3 in the gastrointestinal tissue of the animal model of the syndrome of dampness incumbering middle energizer may be one of the molecular basis in this syndrome.The expressed intervention on distribution of AQP3 by Pingwei Powder in the model may be one of the molecular basis that Pingwei Powder could treat the syndrome.