| HPLC法测定三果汤中没食子酸和鞣花酸的含量 |
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| 引用本文： | 张海伟,罗尚华,杨继佳,范刚,张艺. HPLC法测定三果汤中没食子酸和鞣花酸的含量[J]. 辽宁中医杂志, 2012, 0(12): 2460-2462 |
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| 作者姓名： | 张海伟 罗尚华 杨继佳 范刚 张艺 |
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| 作者单位： | 成都中医药大学民族医药学院 |
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| 基金项目： | 国家自然科学基金项目(30973957,30960507);高校博士点专项科研基金项目(20095132110008) |
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| 摘 要： | 目的:采用HPLC法同时测定三果汤中具有抗氧化活性成分没食子酸和鞣花酸含量的方法。方法:采用的色谱柱为welchrom C18色谱柱(50mm×4.60mm,5μm),流动相为乙腈-0.25%甲酸溶液梯度洗脱,流速为0.85mL/min,柱温25℃,检测波长为260nm。结果:没食子酸和鞣花酸分别在0.0500～0.7000mg,0.0502～0.7140mg范围内呈良好线性,平均回收率分别为103.22%,97.49%(n=6)。结论:采所建方法适用于同时测定三果汤中没食子酸和鞣花酸的含量。
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| 关 键 词： | 藏药 三果汤 没食子酸 鞣花酸 含量测定 |
Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Sanguo Decoction by HPLC |
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| ZHANG Hai-wei,LUO Shang-hua,YANG Ji-jia,FAN Gang,ZHANG Yi. Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Sanguo Decoction by HPLC[J]. Liaoning Journal of Traditional Chinese Medicine, 2012, 0(12): 2460-2462 |
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| Authors: | ZHANG Hai-wei LUO Shang-hua YANG Ji-jia FAN Gang ZHANG Yi |
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| Affiliation: | (College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,Sichuan,China) |
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| Abstract: | Objective:To develop RP-HPLC for the determination of antioxidant contents including gallic acid and ellagic acid in Sanguo Decoction.Methods:The separation was performed on a welchrom C18(50mm×4.60mm,5μm)column with gradient elution.The mobile phase was acetonitrile-0.25% methanoic acid solution,and the flow rate was 0.85mL/min.The detection wavelength was set at 260nm.Billand column temperature was set at 25℃.Results:The linear ranges of gallic acid and ellagic acid were within 0.0500~0.7000mg and 0.0502~0.7140mg,respectively.The average recoveries for gallic and ellagic acid were 103.22%,97.49%(n=6).Conclusion:This method is suitable for the determinination of gallic acid and ellagic acid in Sanguo Decoction. |
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| Keywords: | Tibetan medicine Sanguo Decoction gallic acid ellagic acid determination |
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