探讨针刺水沟和内关对局灶性脑缺血再灌注大鼠PI3K/Akt信号通路的影响

目的:了解局灶性脑缺血再灌注后大鼠脑内PI3K、p-Akt及Caspase3的动态变化,以及针刺水沟、内关对其表达的影响。方法:采用改良的线栓法制备大脑中动脉阻塞1h,不同再灌注时间段(6h、1天、3天、5天、7天)的大鼠短暂局灶性脑缺血模型。应用TUNEL测定不同时点神经细胞凋亡情况,免疫组化和western blot测定不同时点蛋白的表达情况。结果:脑缺血再灌注后1天凋亡细胞百分率、PI3K、p-Akt水平达到峰值,而针刺水沟、内关组凋亡细胞百分率略低于同时点的对照组,相应蛋白表达水平较相同时点对照组增强;在灌注后3天Caspase3达到高峰,而针刺水沟和内关组其表达水平较对照组降低。结论:针刺水沟、内关的神经保护作用可能与PI3K/Akt信号通路有关。

Effect of Needling Shuigou and Neiguan on PI3K/Akt Signaling Pathway in Focal Cerebral Ischemia/Reperfusion Rats

Objective：To explore the changes of PI3K,p-Akt and Caspase3 and the effect of needling Shuigou（DU26）and Neiguan（PC6）on it after the focal ischemia/ reperfusion.Methods：The model of ischemia/reperfusion in rats was made by middle cerebral artery occlusion.Adult Sprague-Dawley rats received occlusion of the left middle cerebral artery for 60min then reperfusion was made for 6h,1d,3d,5d,7d respectively.The apoptotic cells were assessed by TUNEL method.The expressions of PI3K,p-Akt and Caspase3 were evaluated by western blotting at different time points.Results：The percentage of apoptotic cells,the expressions of PI3K and p-Akt reached the peak one day after the reperfusion,while that of Caspase3 reached the peak three days after the reperfusion.Needling Shuigou（DU26）and Neiguan（PC6）made the expressions of PI3K and p-Akt higher,but expression of Caspase3 lower than that in the control groups at different time points.Conclusion：The neuroprotective effect of needling Shuigou（DU26）and Neiguan（PC6）may be related to the PI3K/Akt signaling pathway.