2006-2008年江苏地区不同宿主感染空肠弯曲菌及其耐药性

目的检测2006-2008年江苏地区鸡、水禽、奶牛、丹顶鹤、猪、实验猴等标本感染空肠弯曲菌及其耐药性。方法采用APICampy System进行生化与分类鉴定,结合弯曲菌多重PCR检测方法对来自江苏地区的禽、牛、猪、猴3 010份标本,检测分离株及其耐药性。结果共检测样本3010份,402份空肠弯曲菌阳性,阳性率13.36%,其中家禽样阳性率15.83%（258/1630）;水禽10.40%（52/500）;奶牛7.65%（39/510）;猪18.75%（30/160）;猴15.00%（15/100）;丹顶鹤12.50%（10/80）;糜鹿0%（0/30）。选择不同宿主来源的108株对10大类27种抗生素高度敏感率的是：氯霉素100%、阿莫西林99.07%、阿米卡星92.59%、头孢噻肟91.67%、阿齐霉素90.74%;高度耐药率的是：头孢哌酮99.07%、复方新诺明99.07%、头孢孟多99.07%、磺胺甲基异噁唑97.22%、头孢拉丁91.67%。结果显示,108株分离株的耐药主要集中在9耐～20耐,占85.19%,猪分离株的多重耐药性较其它宿主来源分离株严重。结论江苏地区空肠弯曲菌的流行和耐药状况呈现多样化和复杂化，不同宿主来源分离株耐药性和多重耐药性存在差异。

Susceptibility of different species of animal hosts to Campyelobacter jejuni infection and its drug-resistance

From 2006 to 2008, the susceptibility of different species of animal hosts to Carnpyebacter jejubni infection was observed in various areas of Jiangsu province, in which the API Campy System was used to perform the biochemical identification and the multiple PCR assay was employed to analyse the C. jejuni isolates from 3010 specimens of fouls. Cattle, pigs and monkeys, and in addition, the susceptibility of isolates to various antibiotics was also determined. In these specimens investigated 402 samples were found to be positive in the detection of C. jejuni with a positive detection rate of 13.36%. The positive detection rates in chicken, water fouls, milk cows, pigs, monkey, red crowned crane and wapiti were 15.83%（258/1630）, 10. 4%（52/100）, 8.24%（42/510）, 15. 63%（25/160）, 15% （15/100）, 12.5%（15/80） and 0% （0/30） respectively. Meanwhile, the antibiotics to which the isolates from different hosts showed high rate of sensitivity to 27 antibiotics of 10 varieties included： chloromycetin （100%）, Almocylin （99.7%）, amicarcin （92.59 %）, cefprozil （91.67 %）, alchimyein （90.74 %） ; while the antibiotics to which these isolates showed high rate of resistance were compound neoromin （99.7%）, cefoperazone （99.07%）, trimethoprim （97.22%）, cepronatin （91.67%）, cepromondo （99.07%） respectively. It is evident that the susceptibility of different hosts to C. jejuni infection and the status of drug-resistance of the isolates appear to be quite different and more complicated in Jiangsu province.