胰岛素对2型糖尿病大鼠种植体周围骨形成蛋白-2表达的影响

目的探讨胰岛素对2型糖尿病大鼠种植体周围骨形成蛋白-2表达的影响及意义。方法将SD大鼠30只,随机分为3组正常组、糖尿病组、糖尿病+胰岛素组。糖尿病模型采用高糖高脂饮食加小剂量链脲佐菌素诱导建立;种植模型于大鼠胫骨近骺端植入纯钛种植体(直径1.3mm,长度5mm)。糖尿病+胰岛素组每日定时给予胰岛素皮下注射,正常组、糖尿病组注射等量生理盐水。12周后测量血糖水平及体重,拍摄x线片,取胫骨标本,免疫组化法测定种植体周围骨组织骨形成蛋白-2(BMP-2)的表达水平。结果糖尿病+胰岛素组种植体周围BMP-2表达水平高于糖尿病组,差异有统计学意义(P<0.01)。结论胰岛素可使糖尿病大鼠种植体周围BMP-2表达水平提高,可能促进种植体周围的骨形成。

The effects of insulin on bone morphogenetic protein 2 expression in the peri-implant areas with type 2 diabetic rats

【Objective】 To investigate the expression and meaning of the morphogenetic protein-2 that influenced of insulin in type 2 diabetic rat peri-implant bone.【Methods】 20 type 2 diabetic rats were divided into 3 groups normal group,diabetic group and diabetic + insulin group.Type 2 diabetic model rats were fed on high-glucose-high-fat diet and injected by low dose of streptozotocin(STZ).Implant model rats were implanted the titanium implant(1.3mm×5mm).Diabetic+ insulin group inject with insulin everyday.And the diabetic group and normal group inject with equally physiological saline.12weeks later,blood glucose levels and weight of the rats were collected,X rayed.Then the rats were killed tibia and serum were collected.Immunohistochemical technique was used to assess the expression of BMP-2 in bone tissue around the implant.【Results】 BMP-2 in treatment rats peri-implant bone is more than diabetic group.The difference was statistically significant(P<0.01).【Conclusion】 Insulin can improve morphogenetic BMP-2 in type 2 diabetic rat peri-implant bone.,maybe can improve the bone formation around the implant.