TGF-β1、Smad4在慢性非细菌性前列腺炎大鼠模型前列腺组织中的表达及意义

目的:研究慢性非细菌性前列腺炎(CNP)SD大鼠前列腺组织中转化生长因子(TGF-β1)和Smad4的表达,并初步探讨前列腺组织纤维化的发病机制及意义。方法:将60只6月龄SD大鼠随机分为对照组(假手术)、30dCNP模型组和45dCNP模型组,每组20只。CNP模型组采用去势+高剂量苯甲酸雌二醇肌注方法建立CNP大鼠模型。用免疫组化和免疫印迹方法检查TGF-β1、Smad4在前列腺组织中的表达变化。结果:两个CNP模型组TGF-β1表达显著高于对照组(P均<0.05),而且45dCNP模型组高于30dCNP模型组(P<0.05)。两个模型组Smad4表达显著低于对照组(P均<0.05),而且45dCNP模型组低于30dCNP模型组(P<0.05)。TGF-β1、Smad4在CNP大鼠模型前列腺组织中表达呈负相关。结论:TGF-β1、Smad4可能参与CNP发病过程,前列腺纤维化可能是导致临床难以治愈的重要因素。

Expressions of transforming growth factor-beta(1) and Smad4 in rat models of chronic nonbacterial prostatitis and their clinical significance

Objective:To investigate the expressions of transforming growth factor-beta(1) and Smad4 in the prostatic tissue of rat models of chronic nonbacterial prostatitis (CNP),and to explore the mechanisms of CNP and its fibrosis.Methods:Sixty 6-month-old SD rats were randomly allocated into three groups of equal number:normal control,30 d CNP model and 45 d CNP model,the models made by castration + high-dose intramuscular injection of estradiol benzoate.The expressions of TGF-β1 and Smad4 in the prostatic tissue were detected by immunohistochemistry and Western blot.Results:Compared with the normal controls,the 30 d and 45 d CNP rat models showed a significantly increased expression of TGF-β1 and decreased expression of Smad4 (P<0.05),even more significantly in the 45 d than in the 30 d group.And the expression of TGF-β1 was negatively correlated with that of Smad4 in the CNP rat models.Conclusion:TGF-β1 and Smad4 may be involved in the pathogenesis of CNP,and prostatic fibrosis may make the condition difficult to cure.