全反式维甲酸对人胃腺癌细胞中Akt及其磷酸化蛋白表达的影响

目的研究全反式维甲酸（alltrans-retinoic acid，ATRA）对人胃腺癌细胞（SGC-7901）中Akt及其磷酸化蛋白p—Akt（Ser473）和p—Akt（Thr308）表达的影响及其对细胞凋亡的诱导作用。方法采用四甲基偶氮唑盐（MTT）方法检测ATRA对SGC-7901增殖的抑制作用；荧光混微镜观察细胞凋亡形态；Western blot方法检测不同浓度ATRA处理后的SGC-7901细胞中Akt、p-Akt（Ser473）和p-Akt（Thr308）的表达情况。结果ATRA对SGC-7901细胞增殖的抑制作用呈时间和剂量依赖性；ATRA处理48h时，荧光显微镜观察细胞呈现明显的凋亡形态学改变；Western blot检测显示ATRA对细胞中Akt蛋白的表达没有明显影响，但显著下调两种p-Akt蛋白的表达。结论ATRA诱导SGC～7901细胞凋亡可能与其抑制磷酸化Akt蛋白表达有关。

Effects of all trans-retinoic acid on the expression of Akt and P-Akts in human gastric carcinoma cell line

Objective To investigate the effeets of all trans -retinoic acid（ ATRA） on the expression of Akt or its phosphorylated proteins and on apoptosis of human gastric carcinoma cell line（ SGC -7901 ）. Methods The effects of ATRA on the cell proliferation was measured by methyl thiazolyl tetrazolium（MTT） ,the apoptosis of ATRA on SGC- 7901cells was observed by fluorescentmieroscope, and the expression of Akt, p- Akt （ Ser473 ） and p - Akt （ Thr308 ） were measured by Western blot. Results ATRA inhibits the proliferation of SGC -7901 cells in a dose and time dependent manner. SGC -7901 cells showing typical apoptosis morphological changes were induced by ATRA at 48 h. ATRA did not influence the expression of Akt, but significantly decreased the expression of p - Akt （ Ser473 ） and p - Akt（ Thr308 ）. Conclution ATRA could induce apoptosis by down - regulating expression of p - Akt（ Ser473 ） and p - Akt（ Thr308 ）.