非小细胞肺癌MMP-2、MMP-9、TIMP-1表达及与预后关系研究

目的　研究非小细胞肺癌中MMP 2、MMP 9、TIMP 1蛋白和mRNA的表达 ,探讨其与淋巴结转移及预后的关系。方法　应用免疫组织化学和原位杂交方法检测非小细胞肺癌患者石蜡包埋肺癌组织中MMP 2、MMP 9、TIMP 1蛋白和mRNA表达。结果　MMP 2、MMP 9、TIMP 1的表达在不同年龄、性别、组织学类型及分化程度间无显著性差异 ,在淋巴结转移与否组间有显著性差异 (P值均 <0 .0 1)。患者术后生存期与淋巴结转移与否、MMP 2及MMP 9的表达强度显著相关 (P值分别为 0 .0 13 0、0 .0 175及 0 .0 0 10 )。MMP 2、MMP 9、TIMP 1mRNA与蛋白表达具有一致性 (P <0 .0 1,P <0 .0 0 5 ,P <0 .0 2 5 )。结论　MMP 2、MMP 9是影响预后的独立指标 ;TIMP 1是影响预后的有意义的指标。

Expression of MMP-2, MMP-9, TIMP-1 and their relationship with prognosis in NSCLC

Objective To study the expression of MMP-2, MMP-9, TIMP-1 proteins and mRNA in NSCLC and to analyse their relations with prognosis. Methods Immunohistochemistry (IHC) and in situ hybridization (ISH) were used to detect the expression of MMP-2, MMP-9, TIMP-1 proteins and mRNA in pa-raffin-embedded NSCLC specimens. Results There were no significant differences among the MMP-2, MMP-9, TIMP-1 expression and age, sex, histological type and differentiation. There was statistical relationship between expression of MMP-2, MMP-9, TIMP-1 and lymph node metastasis. Multivariate Cox model analysis suggested that the survival time was significantly related to lymph node metastasis, expression of MMP-2 and MMP-9. The results of IHC and ISH suggested that the concordant rates of MMP-2, MMP-9, TIMP-1 proteins and mRNA were of statistical significance (P<0.01,P<0.005,P<0.025). Conclusion MMP-2 and MMP-9 are independent factors that affect prognosis, TIMP-1 is an useful parameter to the prognosis of NSCLC.