花椒多酚类化合物对脑卒中后抑郁大鼠脑组织NE、5-HT及MAO的影响

目的探讨花椒多酚类化合物总提取物（ZPPC）抗脑卒中后抑郁作用机制。方法建立脑卒中后抑郁大鼠模型,酶联免疫法检测ZPPC、氟西汀对受试大鼠脑组织去甲肾上腺素（NE）、5-羟色胺（5-HT）含量的影响;采用荧光分光光度法测定ZPPC和吗氯贝胺对受试大鼠脑内单胺氧化酶（MAO）活性的影响。结果与空白对照组比较,模型组NE、5-HT水平显著降低（P〈0.05）,ZPPC高剂量组（200mg/kg）和氟西汀组与模型组比较,NE、5-HT水平均有显著性升高（P〈0.05）;ZPPC中、高剂量组（100mg/kg和200mg/kg）还明显抑制大鼠脑组织MAO-A活性（P〈0.05）。结论 ZPPC组能显著提高脑卒中后抑郁大鼠脑组织NE、5-HT水平,其作用机制可能与抑制脑组织MAO-A活性有关。

Influence of Polyphenol Extract from Zanthoxylum Bungeanum Maxim.on Norepinephrine,5-hydroxytryptamine and Monoamine Oxidase in Brain Tissue of Rats With Post-Stroke Depression

Objective To explore the mechanism of polyphenol extract from Zanthoxylum bungeanum Maxim.（ZPPC） on treating post-stroke depression.Methods Post-stroke depression model of rat was established.ELISA was used to detect the influence of ZPPC and fluoxetine on norepinephrine（NE） and 5-hydroxytryptamine（5-HT） contents in brain tissue and the monoamine oxidase（MAO） activity in rats brain was determined by fluorescence spectrophotometry.Results The levels of NE and 5-HT in brain tissue was significantly lower in model group as compared with control group（P〈0.05）,but dramatically elevated after treatment with high-dose ZPPC（200mg/kg） and fluoxetine（P〈0.05）.The activity of MAO in rats brain was inhibited by intervention of ZPPC with 100 and 200 mg/kg as compared with model group（P〈0.05）.Conclusion ZPPC can upregulate NE and 5-HT in brain tissue of rats with post-stroke depression and the underlying mechanism may involve inhibition of MAO activity.