羟丁酸钠减轻新生大鼠缺血缺氧后脑损伤

目的:观察羟丁酸钠对新生大鼠缺血缺氧后脑损伤的影响。方法:60只新生7dSD大鼠随机分为假手术组、模型对照组、羟丁酸钠3个剂量组采用Rice法制作缺血缺氧性脑损害模型。模型对照组缺血缺氧后即刻腹腔注射生理盐水;羟丁酸钠3个剂量组分别注射用生理盐水稀释的羟丁酸钠50,100和200mg·kg-1·次-1,均每日3次,共7d缺血缺氧21d后每组各取6只大鼠作左侧大脑半球重量、含水量测定;另取6只大鼠观察左侧海马CA1区锥体神经元存活数量。结果:(1)模型对照组大鼠左大脑半球重量低于假手术组、羟丁酸钠50mg·kg-1组,差异有显著意义(P<0.05)。(2)各组左侧大脑半球含水量无显著差异(P>0.05)(3)高倍镜下CA1区海马锥体神经元存活数目,模型对照组为每个高倍视野(26±s7)明显低于假手术组(114±27),P<0.01;羟丁酸钠50mg·kg-1100mg·kg-1和200mg·kg-1组每个视野分别为(61±12),(79±24),(58±18),明显高于模型对照组,P<0.01。结论:羟丁酸钠具有改善缺血缺氧后脑损伤作用。

Sodium oxybate alleviated brain injury of neonatal rats with hypoxic-ischemic insult

AIM: To investigate the effects of sodium oxybate on the brain injury in the neonatal rats with hypoxic-ischemic insult. METHODS: Seven-day old Sprague Dawley (SD) rats pups were randomly assigned to model control group, sham group and three sodium oxybate groups with n=12 in each group. Hypoxic-ischemic brain insult models were created by Rice method. All rats except those of sham group underwent left carotid arteries ligation and followed by 2.0 h of hypoxia (8 % oxygen). Saline was administered by ip ( intraperitoneal ) injection at 2 h after inhaled air (sham groups ) or immediately after reoxygenation ( model control groups ) and three times daily thereafter for 7 d. Also, sodium oxybate was administered by the same methods with the dosages 50,100 and 200 mg·kg -1 for the 3 sodium oxybate groups respectively. The brain injury was evaluated by the weight and water content of the left hemisphere, and the survival number of pyramidal neurons in hippocampus CA1 at 21d after hypoxic-ischemic insult. RESULTS: (1) The left hemisphere weight in the model control group was less than that of sham group and sodium oxybate 50 mg·kg -1 (P<0.05), with significant difference at 21 d after hypoxia. (2) The water content of left hemisphere showed no significant changes among different groups (P>0.05) at 21d after hypoxia. (3) The survival pyramidal neurons in the hippocampatus CA1 in model control group (26±s7) were significantly less than those of sham groups ( 114±27), P<0.01; and those of sodium oxybate 50 mg·kg -1, 100 mg·kg -1, 200 mg·kg -1 groups (61±12), (79±24), (58±18) were significantly hight than that of model control groups, P<0.01. CONCLUSION: Sodium oxybate alleviate the brain injury in the rats with hypoxic-ischemic insult.