利用兔模型研究糖基化对冷藏保存人血小板体内生存的影响

利用兔动物模型研究了糖基化对4℃冷藏保存人血小板体内生存的影响。静脉输注棕榈酸乙酯(0.75g/kg)抑制兔网状内皮系统以建立兔动物模型;应用核素标记法检测血小板生存时间;取浓缩人血小板悬液(2×1012/L),添加5g/L的尿嘧啶二磷酸半乳糖(UDGGal),置4℃冰箱冷藏保存,尔后测定输入兔模型体内的冷藏人血小板的生存率。结果表明:添加UDGGal的人血小板,冷藏保存10天后输入兔模型体内的生存时间显著高于空白对照组(P<0.01),而与新鲜血小板对照组相近(P>0.05);输注兔模型体内2小时时的血小板生存率在新鲜血小板对照组、冷藏糖基化血小板组和冷藏空白对照组分别为(68.9±8.5)%、(65.4±8.0)%和(5.0±2.6)%。结论:尿嘧啶二磷酸半乳糖能保护冷藏保存的人血小板,延长冷藏人血小板在兔模型体内的生存时间。

Evalution of the Effects of Glycosylation on In Vivo Survival of Cold-storage Human Platelets by Using Rabbit Model

To study the effects of glycosylation on survival of cold-storage human platelets by using rabbit model. (51)Cr-labeling platelets were used to detect the platelet storage survival. The human platelets (2.0 x 10(12)/L) treated with 5 g/L uridine diphosphate galactose (UDP-Gal) were stored in 4 degrees C refrigeratory up to 10 days. The survival of human platelets in rabbits whose reticuloendothelial system was inhibited by the administration of ethyl palmitate was monitored in blood drawn at various times after the platelet transfusion. The results showed that the survival rate of platelets was significantly increased in cold-storage human platelets by UDP-Gal treatment. The survival rates of platelets at 2 hours after transfusion into rabbits in groups of fresh platelets group, UDP-Gal + cold platelets group and cold platelets group were (68.9 +/- 8.5)%, (65.4 +/- 8.0)% and (5.0 +/- 2.6)%, respectively. Compared with cold platelets group, significant differences were seen among all groups (P < 0.01). UDP-Gal + cold platelets group had no significant differences compared with fresh platelets group (P > 0.05). It is concluded that UDG-Gal can provide the protective effect on cold-storage human platelets and prolong the survival time of refrigerated human platelets in rabbit model.