Persistent Oxidative Stress in Patients with Chronic Active Hepatitis-C Infection After Antiviral Therapy Failure |
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| Authors: | Ghada El-Kannishy Mona Arafa Ibrahim Abdelaal Mohamed Elarman Rasha El-Mahdy |
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| Affiliation: | Department of Internal Medicine, Faculty of Medicine, Mansoura University, Egypt;1Department of Tropical Medicine, Faculty of Medicine, Mansoura University, Egypt;2Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Egypt;3Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt |
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| Abstract: | Background/Aims:Oxidative stress and hepatocellular pathological changes are common associations with chronic hepatitis C virus (CHC) disease. The aim of this study was to assess serum antioxidant-oxidant (Redox) balance in patients with CHC infection before and after intake of the traditional antiviral therapy (pegylated interferon α-2b and oral ribavirin).Patients and Methods:Blood samples from 50 biopsy-proven CHC patients, with no prior anti-viral treatment and persistently elevated serum transaminase levels for 6 months, as well as 15 age- and sex-matched healthy subjects were used for determination of the antioxidants: reduced glutathione (GSH), superoxide dismutase (SOD), α tocopherol and ascorbic acid as well as lipid peroxidation (LPO) index (malondialdehyde [MDA]). The measurements were repeated in the diseased group 25 weeks after pegylated interferon α-2b and ribavirin combination therapy.Results:Serum levels of bilirubin, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were significantly higher in CHC patients than in the control group (P < 0.05). Pretreatment serum MDA values were significantly higher in patients with CHC infection than the control group (P < 0.001), while serum antioxidant levels were significantly lower (P < 0.001). Responders (10 patients) had lower pretreatment serum levels of MDA than non-responders (35 patients) (P < 0.001). Both groups were comparable for the antioxidant serum levels. There was significant negative correlation between serum MDA and serum SOD, GSH, α tocopherol, and ascorbic acid concentrations in CHC patients. On the other hand, there was no correlation between the studied parameters and serum bilirubin, albumin, ALT, and AST.Conclusions:Redox imbalance was detected in patients with CHC. Responders had significantly lower levels of MDA than non-responders. Serum MDA may be used as a pretreatment predictor of response to antiviral treatment in patients with CHC. |
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| Keywords: | Ascorbic acid glutathione lipid peroxidation malondialdehyde oxidative stress superoxide dismutase α tocopherol |
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