不同铁含量饲料对镉致大鼠肾毒性影响

目的 探讨铁含量对镉肾毒性影响。方法 将100只清洁级雄性SD大鼠随机分成低铁、常铁和高铁组,建立低铁、常铁和高铁动物模型,各组再分别设置对照组和染镉组,测定肾损伤指标和尿、肝及肾组织中镉含量。结果 随大鼠体内铁含量的增加,染镉组肾损伤加重;低、常、高铁染镉组尿乙酰-β-D-氨基葡萄糖苷酶(UNAG)和尿β₂微球蛋白(Uβ₂-MG)分别为(119.04±82.4),(220.10±167.77),(347.62±156.18)U/(g.肌酐)和(35.02±18.0),(107.69±27.7),(101.08±48.8)μg/(g.肌酐),组间差异有统计学意义(P<0.05)。结论 在非消化道染镉情况下,机体铁水平增高促进镉对肾脏毒性作用。

Effects of iron in feed on cadmium nephrotoxicity in rats

Objective To explore the effects of different levels of iron in feed on cadmium nephrotoxicity in rats.Methods One hundred clearing grade male SD rats were randomly divided into three groups:iron deficiency diet group, normal iron diet group,and higher level of iron diet group in order to establish anmi almodels of low,normal,and high iron. Each group was divided aga in into control and expermient group.The indicators of iron status,renal injury and cadmium in urine,liver and kidney tissue in each group were detected.Results With the increase of iron load in the body,there was an accrescent tendency in renal damage in the rats of the expermient groups.The activitiy of urine N-acetyl-B-D-gluco sam inides and the content of urine β₂-microglobulin in iron deficiency group,normal iron group and higher level iron group were 119.04·82.4,220.10·167.77,347.62·156.18 U/gcr and 35.02·18.0,107.69·27.7,1011 08·48.8 ug/gcr,respec tively,with significant differences among the groups(P < 0.05).Conclusion Under non-digestive cadmium exposure,dif ferent iron levels canaffect cadmium nephro to xicity.The increase of iron contents promotes cadmium nephro toxicity in rats.