缬草对慢性应激导致的抑郁大鼠大脑海马p-CREB阳性神经元数量的影响

目的探讨最佳剂量缬草对慢性应激导致的抑郁大鼠行为及其大脑海马磷酸化cAMP反应元件结合蛋白(phosphorylated cAMP responsive element-binding protein,p-CREB))阳性神经元的影响。方法将35只大鼠随机均分为正常对照组、未用药模型组、阴性对照模型组、阳性对照模型组、低剂量缬草模型组、中剂量缬草模型组和高剂量缬草模型组。在用药期间,每周测试大鼠体质量、自来水及1%糖水摄取量1次。用药后,计数大脑海马神经元数量,确定缬草最佳剂量。然后根据前述方法,在灌药结束后灌注和固定所有大鼠。采用中性红染色、免疫组化等方法对大鼠海马p-CREB和脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)阳性神经元进行检测。结果高剂量(100mg·kg-1·d-1)缬草能促使抑郁大鼠1%糖水摄取量恢复至正常水平,还可使抑郁大鼠大脑海马神经元以及p-CREB阳性神经元数量恢复到正常水平。正常对照组大鼠大脑海马神经元的BDNF染色较浅淡,其余各组大鼠大脑海马神经元的BDNF阳性染色没有明显差异。结论本研究高剂量(100mg·kg-1·d-1)缬草可能是治疗慢性应激导致大鼠抑郁症的最佳剂量。缬草可以改善抑郁大鼠的行为活动,恢复大脑海马神经元以及p-CREB阳性神经元数量到正常水平。

Effects of Valerian on number of p-CREB positive neurons in cerebral hippocampus of rats with depression induced by chronic mild stress

Objective To explore the effects of the optimal dosage Valerian on behavior,and phosphorylated cAMP responsive element-binding protein(p-CREB) positive neurons in cerebral hippocampus of rats with depression induced by chronic mild stress.Methods Thirty five rats were randomly divided into 7 groups:normal control group,untreated group,negative control group,positive control group,and low-,medium-and high-dose Valerian-treated groups.During the intragastric administration,the body weight,tap water intake and...