苯作业工人外周血细胞DNA损伤的检测

目的研究苯暴露对苯作业工人外周血细胞DNA断裂损伤的影响,并进一步探讨苯暴露与DNA断裂损伤间的剂量-反应关系。方法用3M有机气体被动式采样器监测个体接苯浓度,计算8小时时间加权平均浓度(8hTWA)和累积接苯浓度。用单细胞凝胶电泳试验检测苯作业工人外周血细胞DNA损伤,观察指标包括Olive尾矩和DNA断裂分级。结果接苯工人(51人)Olive尾矩和DNA断裂分级两个参数,均明显高于对照组(31人)(F=3003,P<00001;χ2=2399,P<00001),并均与TWA浓度和累积浓度有剂量-反应关系。Olive尾矩与TWA浓度和累积浓度显著正相关。结论苯暴露导致外周血细胞DNA断裂损伤增加,且呈明显剂量-反应关系,累积接苯浓度比单纯苯浓度更能反映苯暴露水平。

Detection of DNA damages of peripheral white blood cells in benzene-exposed workers

OBJECTIVE: To investigate the effect of benzene exposure on DNA damage of the peripheral white blood cells and to assess the possible dose-response relationship between benzene and DNA damage. METHODS: Personal benzene exposure was sampled with 3M organic vapor monitors. The time weighted average concentration (8h-TWA) and the cumulative dose were calculated. Single cell gel electrophoresis assay was used to detect DNA damage in white blood cells of benzene-exposed workers. The Olive tail moment and the grade of DNA breakage were used to measure DNA damage. RESULTS: The Olive tail moment and the grade of DNA breakage in benzene exposure groups were significantly increased in comparison with those in the control group (F = 30.03, P < 0.0001, chi2 = 239.9, P < 0.0001, respectively) and showed a dose-response relationship with benzene concentration. Correlation analysis showed that Olive tail moment was correlated with benzene exposure concentration. CONCLUSIONS: Benzene exposure resulted in an increase of DNA damage of the peripheral white blood cell, which was in a dose-response relationship manner; cumulative dose was better than simply concentration to reflect benzene exposure.