乌司他丁两种给药途径治疗犬急性出血坏死性胰腺炎

目的 探讨乌司他丁(UTI)外周静脉应用和区域动脉灌注两种给药途径对犬急性出血坏死性胰腺炎(AHNP)的治疗效果.方法 制备犬AHNP模型,随机分为3组.A组:生理盐水(NS)胃十二指肠动脉灌注;B组:UTI+NS外周静脉注入;C组:UTI+NS胃十二指肠动脉灌注.在不同时间段抽血,测血清淀粉酶、TNF-α含量.测胰腺...

Study on two administration ways with UTI in the treatment of acute hemorrhagic necotizing pancreatitis

Objective To probe the therapeutic efficiency by peripheral vein or regional intra-arterial infusion of ulinastatin(UTI) for acute hemorrhagic necotizing pancreatitis(AHNP) in dogs.Methods The mode of AHNP in dogs was prepared and randomly divided into three groups.Group A: infuse NS via gastroduodenal artery;group B: infuse UTI and NS via peripheral vein;Group C: infuse UTI and NS via gastroduodenal artery.Blood sample were taken for the measurement of amylase and TNF-α in the venous blood.The mRNA expression of TNF-α,IL-6 and ICAM-1 in the pancreatic tissue was measured.Pathological changes in the pancreatic tissue were observed under optical microscope and transmission electron microscope.Results ①The concentration of serum amylase of each group after being modeled was much higher than that before.Compared with group A,group B and C reduced the concentration of serum amylase more,and group C had the most remarkerable effectiveness.②The concentration of TNF-α in the venous blood of each group after being modeled were much higher than that before.In group B,TNF-α was reduced at partial time;but in group C which was reduced all the time.③In group B and C,the mRNA expression of TNF-α,IL-6 and ICAM-1 in the pancreatic tissue were down-regulated,and group C had the most remarkerable effectiveness.④Group C had the most slight pathological change in pancreatic tissue under optical microscope and transmission electron microscope.Conclusion Regional intra-arterial infusion UTI early has the favourable treament effect on AHNP.Its therapeutic efficiency is better than that by peripheral vein.