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| 人牙周膜细胞接种于纳米-羟基磷灰石上的形态学研究 | |
| 引用本文： | 毛钊,毛曦,储成林.人牙周膜细胞接种于纳米-羟基磷灰石上的形态学研究[J].东南国防医药,2008,10(3):161-163. |
| 作者姓名： | 毛钊 毛曦 储成林 |
| 作者单位： | 1. 南京军区南京总医院口腔科,江苏南京,210002 2. 南京大学生命科学院,江苏南京,210093 3. 东南大学材料研究所,江苏南京,210096 |
| 基金项目： | 江苏省高技术研究发展计划项目 , 南京军区医药卫生基金 |
| 摘 要： | 目的观察人牙周膜细胞接种于三维多孔纳米-羟基磷灰石上的组织形态学表现。方法将原代培养的人牙周膜细胞接种于三维多孔纳米-羟基磷灰石上，培养15天后，采用扫描电子显微镜观察细胞的生长情况，探讨两者的复合情况。结果作为种子细胞的人牙周膜细胞接种到支架材料上后，细胞粘附充分，生长旺盛，并有钙结节和少量胶原纤维形成。结论三维多孔纳米羟基磷灰石支架材料是一种比较符合牙周组织工程学条件的支架材料。 |
| 关 键 词： | 人牙周膜细胞 三维多孔纳米-羟基磷灰石支架材料 牙周组织工程 |
| 文章编号： | 1672-271X(2008)03-0161-03 |
| 修稿时间： | 2008年3月25日 |
The study on morphology by seeding human periodontal ligament cells onto three-dimensional porous nano-hydroxylapatite |
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| MAO Zhao,MAO Xi,CHU Cheng-lin.The study on morphology by seeding human periodontal ligament cells onto three-dimensional porous nano-hydroxylapatite[J].Journal of Southeast China National Defence Medical Science,2008,10(3):161-163. | |
| Authors: | MAO Zhao MAO Xi CHU Cheng-lin |
| Institution: | MAO Zhao ,MAO Xi ,CHU Cheng-lin (1. Department of Stomatology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, Jiangsu, China; 2. Department of Biological Science and Technology, School of Life Sciences, Nanjing University, Nanjing 210093, Jiangsu, China; 3. Institute of Functional Materials, Southeast University, Nanjing 210096, Jiangsu, China) |
| Abstract: | Objective To observe the morphology by seeding human periodontal ligament cells onto three-dimensional porous nano hydroxylapatite. Methods Human periodontal ligament cells were seeded onto three-dimensional porous nano-hydroxylapatite scaffolds,cultured for 15 days in vitro. Scanning electron microscopy was used to observe morphology of cells seeded on threedimensional porous nano-hydroxylapatite scaffolds. Results The cells seeded on three-dimensional porous nano-hydroxylapatite scaffolds had sufficient adhesive behavior,proliferated well and produced many tiny calcium nodules and collagenous fibers. Conclusion The study demonstrats that threedimensional porous nano-hydroxylapatite scaffolds may be considered as a suitable scaffold in periodontal tissue engineering. |
| Keywords: | Human periodontal ligament cells Three-dimensional porous nano-hydroxylapatite scaffolds Periodontal tissue engineering |
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