EB病毒感染与hMLH1蛋白表达在子宫颈癌发病中的意义

目的:了解宫颈癌Epstein-Barr病毒(EBV)感染情况及错配修复基因hMLH1表达在宫颈癌发生、发展中的意义。方法:采用免疫组织化学SP法检测临床资料较完整的72例子宫颈鳞癌和31例慢性宫颈炎组织EBV潜伏期膜蛋白(LMP1)与hMLH1蛋白表达,并分析二者的相关关系。结果:在宫颈炎及宫颈癌组EBV(LMP1)的表达率分别为90·3%(28/31)和69·4%(50/72),差异显著(P<0·05);hMLH1的表达率分别为61·3%(19/31)和58·3%(42/72),无显著差异(P>0·05);EBV感染组和非EBV感染组hMLH1的表达率分别为67·5%(54/80)和43·5%(10/23);差异显著(P<0·05);EBV(LMP1)和hMLH1的表达率与宫颈癌的分级、分期及淋巴结转移均无明显相关关系(P>0·05);与是否绝经无明显相关关系(P>0·05)。结论:宫颈炎和宫颈癌EBV感染率均较高,宫颈炎患者宫颈上皮EBV感染可能为致宫颈癌的高危因素,有待于进一步证实。EBV感染时hMLH表达率增高,提示EBV感染引起的宫颈细胞基因突变增多可能为EBV的致癌机制之一。

Significance of EBV infection and hMLHI protein expression in cervical cancer pathogenesis

Objective:To investigate the significance of EBV infection and mismatch repair(MMR)gene hMLH1 protein expression in cervical carcinoma pathogenesis and development.Methods:It was performed immunohistochemical analyses of formalin fixed,paraffin-embedded 31chronic cervical inflammation(CCI)biopsies and 72 squamous cervical carcinoma(SCC)samples to detect EBV(LMP1)and hMLH1 protein expressions.Results:EBV(LMP1)expressed in 90.3%(28/31)of CCI and 69.4%(50/72)of SCC,(P<0.05);hMLH1 expressed in 61.3%(19/31)of CCI and 58.3%(42/72)of SCC(P>0.05);hMLH1expressed in 67.5%(54/80)in EBV( )group and 43.5%(10/23)in EBV(-)group,(P<0.05);There were no correlations between EBV infection and hMLH1 protein expression with the grade,stage,lymph node involvement and whether the patients were in menopause in SCC,(P>0.05).Conclusions:EBV infection rates of CCI and SCC are very high,EBV infection may be involved in the development of cervical cancer,although further study should be performed to elucidate a causative role of EBV for the cancer.EBV infection may induce gene mutation of cervical epithelial increased,which played an important role in cervical carcinogenesis.