程序升温气相色谱法测定樟脑乳膏中主药的含量

目的:建立以程序升温气相色谱法测定樟脑乳膏中主药含量的方法。方法:色谱柱为ATSE-54石英毛细管柱;程序升温中初始温度60℃,维持时间5min,升温速度为10℃·min-1,最终温度180℃,维持时间5min;进样口温度为130℃;氢火焰离子化检测器温度为215℃;进样量为2μL。水浴加热下用甲醇溶解樟脑乳膏,冰浴,过滤后直接进样测定。结果:樟脑检测浓度线性范围为10～120μg·mL-1(r=0.9998),平均回收率为101.41%～103.04%,RSD<0.56%。结论:该方法简单、准确、快速,适用于控制樟脑乳膏中主药的含量。

Content Determination of Main Component in Camphor Cream by Programmed Temperature GC

OBJECTIVE： To determine the content of main component in camphor cream by programmed temperature GC. METHODS： The determination of sample was performed on AT SE - 54 quartz capillary column; programmed temperature： the initial temperature was kept at 60℃ for 5 minutes with a temperature rising rate at 10℃·min^-1, and the terminal temperature was kept at 180℃ for 5 rain; the temperature at injector was kept at 130℃; the temperature of FID detector was kept at 215℃; the sample size was 2μL. Camphor cream was placed in hot water - bath and dissolved by methanol, which was then placed in icebath for filtration and determination. RESULTS： The linear concentration range of camphor was 10-120μg·mL^-1（r=0.999 8） and its average recovery rate was 101.41%-103.04% （RSD〈0.56%）. CONCLUSION： The method is simple, accurate and rapid, and it is applicable for the control of the concentration of main component in camphor cream.