头状蓼提取物体外抗氧化活性研究 |
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引用本文: | 吴彩霞,;刘瑜新,;常星,;康文艺.头状蓼提取物体外抗氧化活性研究[J].中国药房,2009(24):1845-1847. |
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作者姓名: | 吴彩霞 ;刘瑜新 ;常星 ;康文艺 |
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作者单位: | [1]河南大学天然药物研究所,开封市475004; [2]河南省疾病预防控制中心,郑州市450003 |
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基金项目: | 河南省教育厅自然科学研究计划项目(2008A360002);河南省教育厅高等学校青年骨干教师资助计划(2008-23) |
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摘 要: | 目的:研究头状蓼提取物的体外抗氧化活性。方法:以脂溶性维生素E及二丁基羟基甲苯(BHT)作对照,采用清除二苯代苦味酰基(DPPH)自由基、清除2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基和铁离子(Fe3+)还原/抗氧化能力(FRAP)测定法检测头状蓼石油醚、乙酸乙酯、甲醇提取物的体外抗氧化活性。结果:与头状蓼石油醚、乙酸乙酯提取物比较,头状蓼甲醇提取物清除DPPH自由基、清除ABTS自由基及还原Fe3+的能力较强。结论:头状蓼甲醇提取物具有较好的体外抗氧化活性。3种方法中,DPPH方法和ABTS方法相关性最高。
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关 键 词: | 头状蓼 体外 抗氧化 二苯代苦味酰基 2 2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐 铁离子还原/抗氧化能力法 |
In Vitro Antioxidant Activity of Extracts from Polygonum alatum |
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Institution: | WU Cai-xia, LIU Yu-xin, CHANG Xing, KANG Wen-yi, WU Cai-xia, KANG Wen-yi(1.Institute of Natural Products, Henan University, Kaifeng 475004, China;2.Henan Center for Disease Control and Prevention, Zhengzhou 450003,China) |
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Abstract: | OBJECTIVE: To study the in vitro antioxidant activity of the extracts from Polygonum alaturn .METHODS: 2, 2 - diphenyl - 1 - picrylhydrazyl assay(DPPH), 2, 2' - amino - di(3 - ethyl - benzothiazoline sulphonic acid - 6) ammonium salt assay(ABTS) and ferric reducing antioxidant power assay(FRAP) were employed to analyze the antioxidant activity of extracts(ligarine extract, acetic ether extract and methanolic extract) from P.alatum with lipid- soluble vitamin and dibenzylatiooluene(BHT) as controls.RESULTS: Compared with ligarine extract and acetic ether extract, the methanol extract of P.alatum showed higher potency in scavenging DPPH radical and ABTS radical and reducing Fe^3+ .CONCLUSION: The methanol extract of P.alatum showed high antioxidant activity in vitro. Among the 3 methods, the closest correlation was noted between DPPH assay and ABTS assay. |
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Keywords: | Polygonum alarum In vitro Antioxidant activity DPPH ABTS FRAP assay |
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