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头状蓼提取物体外抗氧化活性研究
引用本文:吴彩霞,;刘瑜新,;常星,;康文艺.头状蓼提取物体外抗氧化活性研究[J].中国药房,2009(24):1845-1847.
作者姓名:吴彩霞  ;刘瑜新  ;常星  ;康文艺
作者单位:[1]河南大学天然药物研究所,开封市475004; [2]河南省疾病预防控制中心,郑州市450003
基金项目:河南省教育厅自然科学研究计划项目(2008A360002);河南省教育厅高等学校青年骨干教师资助计划(2008-23)
摘    要:目的:研究头状蓼提取物的体外抗氧化活性。方法:以脂溶性维生素E及二丁基羟基甲苯(BHT)作对照,采用清除二苯代苦味酰基(DPPH)自由基、清除2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基和铁离子(Fe3+)还原/抗氧化能力(FRAP)测定法检测头状蓼石油醚、乙酸乙酯、甲醇提取物的体外抗氧化活性。结果:与头状蓼石油醚、乙酸乙酯提取物比较,头状蓼甲醇提取物清除DPPH自由基、清除ABTS自由基及还原Fe3+的能力较强。结论:头状蓼甲醇提取物具有较好的体外抗氧化活性。3种方法中,DPPH方法和ABTS方法相关性最高。

关 键 词:头状蓼  体外  抗氧化  二苯代苦味酰基  2  2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐  铁离子还原/抗氧化能力法

In Vitro Antioxidant Activity of Extracts from Polygonum alatum
Institution:WU Cai-xia, LIU Yu-xin, CHANG Xing, KANG Wen-yi, WU Cai-xia, KANG Wen-yi(1.Institute of Natural Products, Henan University, Kaifeng 475004, China;2.Henan Center for Disease Control and Prevention, Zhengzhou 450003,China)
Abstract:OBJECTIVE: To study the in vitro antioxidant activity of the extracts from Polygonum alaturn .METHODS: 2, 2 - diphenyl - 1 - picrylhydrazyl assay(DPPH), 2, 2' - amino - di(3 - ethyl - benzothiazoline sulphonic acid - 6) ammonium salt assay(ABTS) and ferric reducing antioxidant power assay(FRAP) were employed to analyze the antioxidant activity of extracts(ligarine extract, acetic ether extract and methanolic extract) from P.alatum with lipid- soluble vitamin and dibenzylatiooluene(BHT) as controls.RESULTS: Compared with ligarine extract and acetic ether extract, the methanol extract of P.alatum showed higher potency in scavenging DPPH radical and ABTS radical and reducing Fe^3+ .CONCLUSION: The methanol extract of P.alatum showed high antioxidant activity in vitro. Among the 3 methods, the closest correlation was noted between DPPH assay and ABTS assay.
Keywords:Polygonum alarum  In vitro  Antioxidant activity  DPPH  ABTS  FRAP assay
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