水芹乙酸乙酯提取物对大鼠心肌缺血和缺血再灌注损伤的保护作用研究

目的:研究水芹乙酸乙酯提取物对大鼠心肌缺血和缺血再灌注损伤的保护作用。方法:使用线栓法复制急性心肌缺血和缺血再灌注损伤模型。缺血前5min静脉注射水芹乙酸乙酯提取物,动态观察模型大鼠心电图的变化,测定心肌缺血引起的心律失常和心肌梗死面积、心肌缺血再灌注引起心律失常情况、血浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:水芹乙酸乙酯提取物可显著对抗心肌缺血引起的心律失常和缩小心肌梗死面积;可显著对抗心肌缺血再灌注损伤引起的心律失常,提高血浆中SOD活性和降低MDA含量。结论:水芹乙酸乙酯提取物对大鼠心肌缺血和缺血再灌注损伤具有保护作用,其机制可能与抗过氧化作用有关。

Protective Effect of Ethyl Acetate Extract from Oenanthe stolonifera against Myocardial Ischemia and Ischemia-reperfusion Injury in Rats

OBJECTIVE： To study the protective effect of ethyl acetate extract from Oenanthe stolonifera against myocardial ischemia and ischemia reperfusion injury in rats. METHODS： Acute myocardial ischemia and ischemical reperfusion injury models were prepared by suture occluded method. 5 min before the onset of ischemia, the rats were injected with ethyl acetate extract from O.stolonifera, then the dynamic ECG change of rats was observed, and the arrhythmia caused by myocardial ischemia and the myocardial infraction size, the arrhythmia induced by myocardial ischemia - reperfusion, the activity of superoxide dismutase（SOD） and the content of malondialdehyde（MDA） in plasma were determined. RESULTS： Ethyl acetate extract from O. stolonifera significantly inhibited myocardial ischemia - induced arrhythmia and decreased the myocardial infarction size, significantly inhibited arrhythmia induced by myocardial ischemia- reperfusion, enhanced SOD activity and decreased the content of MDA in plasma. CONCLUSION： The protective effect of ethyl acetate extract from O.stolonifera against myocardial ischemia and ischemieal reperfusion injury in rats is possibly associated to its anti - peroxidation effect.