| RP-HPLC法测定银翘解毒丸中连翘苷的含量 |
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| 引用本文： | 郭淑英,郝乘仪,冯波.RP-HPLC法测定银翘解毒丸中连翘苷的含量[J].中国药房,2009(24):1896-1897. |
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| 作者姓名： | 郭淑英 郝乘仪 冯波 |
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| 作者单位： | 吉林医药学院药学院,吉林市132013 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定银翘解毒丸中连翘苷含量的方法。方法:色谱柱为SHIM-PACK VP-ODS(150mm×4.6mm,5μm),流动相为乙腈-水(25∶75),流速为1.0mL.min-1,检测波长为277nm,柱温为30℃。结果:连翘苷进样量在0.453~3.481μg范围内与峰面积积分值呈良好线性关系(r=0.999 8);平均回收率为99.50%,RSD=1.15%(n=6)。结论:本方法简便、快速、准确,可用于银翘解毒丸的质量控制。
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| 关 键 词： | 反相高效液相色谱法 银翘解毒丸 连翘苷 含量测定 |
Determination of Forsythin of Yinqiao Jiedu Pills by RP - HPLC |
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| GUO Shu-ying,HAO Cheng-yi,FENG Bo.Determination of Forsythin of Yinqiao Jiedu Pills by RP - HPLC[J].China Pharmacy,2009(24):1896-1897. |
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| Authors: | GUO Shu-ying HAO Cheng-yi FENG Bo |
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| Institution: | (Department of Pharmacy, Jilin Medical College, Jilin 132013, China) |
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| Abstract: | OBJECTIVE： To estabish a RP - HPLC method for the content determination of forsythin of Yinqiao jiedu pills. METHODS： The determination was performed using SHIM- PACK VP- ODS column（ 150 mm× 4.6 mm, 5μm） with column temperature at 30 ℃. The mobile phase was acetonitril- water（25 ： 75） with flow rate at 1.0 mL · min^-1.The detective wavelength was set at 277 nm. RESULTS： The linear range of forsythin was 0. 453--3.481μg（ r = 0.999 8） . The mean recovery was 99.50% （RSD = 1.15%, n =6） .CONCLUSION： The method was simple, rapid, accurate and suitable for the quality control of Yinqiao jiedu pills. |
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| Keywords: | RP - HPLC Yinqiao jiedu pills Forsythin Content determination |
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