| HPLC法测定前列康欣颗粒中丹酚酸B的含量 |
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| 引用本文： | 张凤芹,龚伟,曲雷鸣.HPLC法测定前列康欣颗粒中丹酚酸B的含量[J].中国药房,2009(33):2614-2615. |
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| 作者姓名： | 张凤芹 龚伟 曲雷鸣 |
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| 作者单位： | 辽宁中医药大学附属医院,沈阳市110032 |
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| 摘 要： | 目的:建立以高效液相色谱法测定前列康欣颗粒中丹酚酸B含量的方法。方法:色谱柱为DiamonsilTMC18(250mm×4.6mm,5μm),流动相为甲醇-乙睛-水-甲酸(25∶10∶64∶1),检测波长为286nm,流速为1.0mL.min-1。结果:丹酚酸B进样量在0.206～1.854μg范围内与峰面积积分值呈良好线性关系(r=0.999 9);平均回收率为98.0%,RSD=1.5%(n=6)。结论:本方法简便可靠、结果稳定、重复性好,可用于前列康欣颗粒的质量控制。
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| 关 键 词： | 高效液相色谱法 前列康欣颗粒 丹酚酸B 含量测定 |
Determination of Salvianolic Acid B in Qianlie Kangxin Granules by HPLC |
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| ZHANG Feng-qin,GONG Wei,QU Lei-ming.Determination of Salvianolic Acid B in Qianlie Kangxin Granules by HPLC[J].China Pharmacy,2009(33):2614-2615. |
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| Authors: | ZHANG Feng-qin GONG Wei QU Lei-ming |
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| Institution: | (The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China) |
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| Abstract: | OBJECTIVE： To develop an HPLC method for the determination of salvianolic acid B in Qianlie kangxin granules. METHODS： The sample was determined on DiamonsilTM C18（250 mm × 4.6 mm, 5μm） with the mobile phase consisted of methanol- acetonitrile-water -formic acid（25 ： 10：64 ： 1） at a flow rate of 1.0 mL . min^-1.The detection wavelength was set at 286 nm. RESULTS： The linear range of salvianolic acid B was 0. 206-1. 854μg（ r = 0.999 9） .The average recovery rate for salvianolic acid B was 98.0% （RSD = 1.5%, n = 6）. CONCLUSION： The established method is simple, reliable, stable in results and reproducible, and it is applicable for the quality control of Qianlie kangxin granules. |
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| Keywords: | HPLC Qianlie kangxin granules Salvianolic acid B Content determination |
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