疏肝健脾方对模型大鼠乳腺增生组织细胞增殖/凋亡的影响

目的:研究疏肝健脾方对模型大鼠乳腺增生组织细胞增殖/凋亡的影响。方法:在采用苯甲酸雌二醇和黄体酮肌肉注射复制模型的同时,灌胃疏肝健脾方30d,观察大鼠乳腺组织增生的程度以及乳腺组织中增殖细胞核抗原(PCNA)、细胞凋亡相关调控因子Bcl-2、Bax的表达和凋亡指数(AI)。结果:疏肝健脾方能够抑制乳腺组织增生,降低PCNA和Bcl-2的表达活性,增加Bax的表达活性和AI,抑制乳腺组织细胞增殖,促进细胞凋亡。结论:疏肝健脾方通过调控大鼠乳腺增生组织细胞增殖/凋亡,阻断/逆转大鼠乳腺组织增生。

Effect of Shugan Jianpi Formula on Proliferation/Apoptosis of Hyperplastic Tissues of Mammary Gland in Model Rats

OBJECTIVE： To explore the effect of Shugan jianpi formula on the proliferation and apoptosis of the hyperplastic tissues of mammary gland of model rats. METHODS： The rats were injected with benzestrofol and progesterone to establish mammoplasia model, meanwhile which were treated intragastrically with Shugan jianpi formula for 30 days. The degree of mammoplasia, as well as the PCNA （proliferating cell nuclear antigen）, the apoptotic related regulating factors such as Bcl-2 and Bax and the apoptotic index （AI） of the mammary gland were detected. RESULTS： Shugan jianpi formula inhibited the mammoplasia, down - regulated the expression levels of PCNA and Bcl - 2, up - regulated the expression level of Bax and AI, inhibited the proliferation of the mammary tissues and promoted apoptosis. CONCLUSION：Shugan jianpi formula blocks or reverses the hyperplasia of mammary gland by regulating the cell proliferation/apoptosis of the hyperplastic tissues of mammary gland.