RP-HPLC法测定比格犬血浆中格列美脲的浓度

目的:建立反相高效液相色谱法测定比格犬血浆中格列美脲(GM)的浓度。方法:色谱柱为Agilent Eclipse XDB-C8,柱温为30℃,流动相为乙腈-甲醇-10mmol·L-1磷酸二氢钾(65∶40∶95,pH7.2),流速为1.0mL·min-1,检测波长为227nm。以吉非罗齐为内标,采用液-液萃取法预处理血浆样品,测定6只比格犬单剂量给予GM舌下喷雾剂(2mg)后不同时间点的血药浓度。结果:GM检测浓度线性范围为50～2500ng·mL-1,最低检出限为20ng·mL-1,日内和日间RSD均小于15%,萃取回收率为71.27%～103.5%,方法回收率为87.97%～98.95%。结论:本方法样品处理简单,灵敏度高,适合分析生物样品中的GM。

Determination of Plasma Concentration of Glimepiride in Beagle Dogs by RP- HPLC

OBJECTIVE： To establish an RP - HPLC method for determination of plasma concentration of glimepiride （GM） in Beagle dogs. METHODS： Plasma sample was separated on Agilent Eclipse XDB- C8 column with column temperature kept at 30 ℃, and the mobile phase consisted of acetonitrile- methanol -- 10 mmol · L-1 potassium dihydrogen phosphate （65 ： 40 ： 95, pH value adjusted to 7.2） at a flow rate of 1.0 mL · min -1. The UV detection wavelength was set at 227 nm. Plasma sample were pretreated by liquid - liquid extaetion with gemfibrozil as an internal standard. Plasma concentrations of GM in 6 Beagle dogs at different time points were determined after a single sublingual dosing of 2 mg GM spray. RESULTS： The linear range of GM was 50--2 500 ng ·mL -1 with a lowest detection limit of 20 ng · mL -1. The intra - day RSD and inter - day RSD were all less than 15%. The extraction recovery rate was 71.27% --103.5%, and the methodological recovery rate was 87.97% -- 98.95%. CONCLUSION： This method is simple in sample treatment and sensitive, and it is applicable for analysis of GM in biological sample.