白芍醇提液超滤膜分离药效部位体外抗肿瘤活性研究

目的:研究超滤膜对白芍醇提液分离的药效部位及其体外抗肿瘤的活性。方法:采用高效液相色谱法测定白芍超滤液中芍药苷的含量;以动物血清培养小鼠HT29、HL60和S180肿瘤细胞,观察白芍超滤液药效部位对肿瘤细胞的抑制作用及与给药量和给药时间的关系。结果:给药剂量在10～100g·kg-1含药血清范围内对各肿瘤细胞均有显著抑制作用,其作用与给药量呈正相关。药物血清在不同时间对各肿瘤细胞均有显著抑制作用,其作用与给药时间呈正相关。结论:超滤膜分离的白芍醇提液药效部位在体外有抗肿瘤活性。

In Vitro Antineoplastic Activity of the Effective Portion of the Ultrafiltration Membrane- separated Ethanol Extract of Paeonia lactiflora

OBJECTIVE ： To study the effective portion of the ultrafiltration membrane - separated ethanol extract of Pae onia lactiflora and its antineoplastic activity in vitro. METHODS： The content of peoniflorin in ethanol extract of P. lactiflora was determined by HPLC. The drug - containing serum of mice was used to cultivate tumor cells such as HT29, HL60 and S180, then the inhibitory effect of P.lactiflora ultrafiltrate on the tumor cells and the association between dosage and administration time of the P.lactiflora ultrafiltrate were observed. RESULTS： P. lactiflora （10-100 g · kg^ -1） showed marked inhibition on all kinds of the above- mentioned tumor cells in a dose- dependent manner; the drug- containing serum also showed marked inhibition on all of kinds of tumor cells at different time in a time - dependent manner. CONCLUSION： The effective portion of ultrafiltration membrane- separated ethanol extract of P.lactiflora has antineoplastie activity in vitro.