何首乌醇提物的分离纯化及活性研究

目的:分离纯化何首乌醇提物的有效部位并对其进行活性研究。方法:采用硅胶柱色谱法对何首乌醇提物的正丁醇萃取物进行分离,用不同浓度的氯仿-甲醇洗脱剂进行梯度洗脱,采用十八烷基硅烷键合硅胶柱和硅胶薄层制备板分离出不同化合物,并采用MTT法测定其对人脐静脉血管内皮细胞生长的作用。结果:分离出3种化合物;不同化合物对人脐静脉血管内皮细胞的生长具有促进、抑制等作用。结论:本方法可用于何首乌醇提物中亲水性成分的分离与纯化;初步发现何首乌中含有能促进人脐静脉血管内皮细胞生长的化合物。

Separation and Purification of the Ethanol Extracts of Polygonum multiflorum and Study on Its Activity

OBJECTIVE： To separate and purify the effective parts of the extracts of Polygonum multiflorum and study the activity of the effective parts. METHODS： The n-butanol extracts of P.multiflorum extracts were separated by silica gel column chromatography and eluted with different concentration of mixture of chloroform- methanol eluant gradiently, then the compounds were isolated by using ODS column and silica gel thin - layer preparation plate, and the effects of the compounds on the growth of human umbilical vein vascular endothelial cells were determined by MTT assay. RESULTS： A total of three compounds were isolated. The compounds could promote or inhibit the growth of human umbilical vein vascular endothelial cells. CONCLUSION： The established method is suitable for the separation and purification of the hydrophilic components of the ethanol extract of P.multiflorum. It is initially found out that the compounds that can promote the growth of human umbilical vein vascular endothelial cell were contained in P. multiflorum.