注射用血栓通(冻干)的HPLC指纹图谱研究

目的:建立注射用血栓通(冻干)的高效液相色谱指纹图谱。方法:色谱柱为Zorbax SB-Aq-C18(250mm×4.6mm,5μm),流动相为乙腈-水(梯度洗脱),检测波长为203nm。结果:确定了注射用血栓通(冻干)的13个共有峰,并得到了以人参皂苷Rg1为内标的相对保留时间及峰面积比值,非共有峰面积未超过总峰面积的5%。结论:本方法简单、可靠,可用于注射用血栓通(冻干)的质量控制。

HPLC Fingerprints of Freeze-dried Xueshuantong for Injection

OBJECTIVE： To establish the HPLC fingerprints of the freeze- dried Xueshuantong for injection. METHODS： The HPLC chromatographic column was Zorbax SB Aq- C18（250 mm × 4.6 mm, 5 μm） and the mobile phase consisted of acetonitrile - water for gradient elution. The UV detection wavelength was set at 203 nm. RESULTS： There were 13 mutual peaks in the HPLC fingerprints of the freeze - dried Xueshuantong for injection. The relative retention time and relative peak area ratio of the fingerprints in which ginsenosides Rg1 served as internal standard were obtained. The area of non mutual peaks represented no more than 5% of total peak area. CONCLUSION： The method is simple and reliable and it is applicable for the quality control of the freeze- dried Xueshuantong for injection.