RP-HPLC法测定人血浆中奥扎格雷的浓度

目的:建立以反相高效液相色谱法测定人血浆中奥扎格雷浓度的方法。方法:色谱柱为SinoChrom OPS-AP,流动相为0.025mol.L-1磷酸二氢钾缓冲液-乙腈(85:15),检测波长为274nm,流速为0.8mL.min-1,内标为左羟丙哌嗪。结果:奥扎格雷血药浓度在0.7～21.3μg.mL-1范围内线性关系良好(r=0.9996);提取回收率在83.78%～89.14%之间,方法回收率在101.95%～106.68之间,日内、日间RSD分别为1.33%～3.27%、2.72%～5.97%。结论:本方法操作简便、快速、准确、重现性好,适用于奥扎格雷的药动学研究。

Determination of Plasma Ozagrel Concentration by RP - HPLC

OBJECTIVE： To determine the concentration of ozagrel in human plasma by RP- HPLC. METHODS： The sample separation was performed on a SinoChrom OPS- AP column. The mobile phase consisted of 0.025 mol·L^-1 potassium dihydrogen phosphate buffer solution-acetonitrile（85 ： 15） with a flow rate of 0.8 mL·min^-1 and a detection wavelength of 274 nm. The internal standard was levodropropizine. RESULTS： The linear range of ozagrel was 0.7-21.3μg·mL^-1, （r = 0.999 6） . The extraction recovery of ozagrel was 83.78%- 89.14% and the methodological recovery of it was 101.95% 106.68%. The intra- day RSD and inter- day RSD were 1.33%--3.27% and 2.72%- 5.97% , respectively. CONCLUSION： This method is simple, rapid,accurate and reproducible, and it is applicable for the pharmacokinetic study of ozagrel in human plasma.