微柱离心-HPLC法测定盐酸丁卡因脂质体包封率

目的:建立测定盐酸丁卡因脂质体包封率的方法。方法:采用微柱离心法分离脂质体与游离药物,以高效液相色谱法测定药物浓度并计算包封率。结果:微柱离心法能很好地将脂质体与游离药物分离,空白脂质体的回收率为90.4%～100.1%,游离药物吸附率为96.6%～99.2%;盐酸丁卡因脂质体包封率均在80%左右。结论:所建盐酸丁卡因脂质体包封率的测定方法简单、快速、重现性好。

Determination of the Entrapment Efficiency of Tetracaine Hydrochloride Liposome by the Microcolumn Centrifugation - HPLC

OBJECTIVE： To develop a method for determining the entrapment efficiency of tetracaine hydrochloride liposome. METHODS： The microcolumn centrifugation was adopted to separate the free tetracaine hydrochloride from liposomes; the content of tetracaine hydrochloride was determined by HPLC and the entrapment efficiency was computed. RESULTS： By microcolumn centrifugation, the tiposomes and the free tetracaine hydroehloride were well -separated. The recovery rate of the blank liposomes was 90.4%- 100.1%; the absorption rate of free tetracaine hydrochloride was 96.6% 99.2% ; and the entrapment efficiencies of tetracaine liposomes stood at about 80%. CONCLUSION： This method established for determining the entrapment efficiency of tetracaine hydrochloride liposome is simple, rapid and reproducible.