| Numb基因在培养神经干细胞中的表达 |
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| 引用本文： | 许汉鹏,苟琳,黄文晋,鞠躬. Numb基因在培养神经干细胞中的表达[J]. 神经解剖学杂志, 2002, 18(4): 293-297,T058 |
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| 作者姓名： | 许汉鹏 苟琳 黄文晋 鞠躬 |
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| 作者单位： | 1. 第四军医大学基础部,神经科学研究所,西安,710032
2. 航空航天医学系,航空航天临床医学教研室,西安,710032 |
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| 摘 要： | 为了观察在体外培养的神经干细胞内参与细胞发育分化和细胞非对称性分裂的 notch信号传导路的分子 numb的分布和表达 ,采用无血清培养技术 ,在体外进行神经干细胞克隆的培养传代 ,经过免疫细胞化学鉴定后 ,用 num b特异性引物对培养的神经干细胞进行 RT-PCR分析 ,PCR产物经克隆测序后 ,用地高辛标记 ,对神经干细胞克隆进行原位杂交。结果表明 :在培养的神经干细胞内有大量细胞 ( >95 % )均为 numb基因表达信号阳性 ;干细胞克隆内部中心和边缘相互间未见明显差别
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| 关 键 词： | numb notch 受体 RT-PCR 细胞培养 神经干细胞 原位杂交 小鼠 |
EXPRESSION OF NUMB GENE IN CULTURED NEURAL STEM CELL |
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| Xu Hanpeng,Gou Lin ,Huang Wenjin,Ju Gong. EXPRESSION OF NUMB GENE IN CULTURED NEURAL STEM CELL[J]. Chinese Journal of Neuroanatomy, 2002, 18(4): 293-297,T058 |
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| Authors: | Xu Hanpeng Gou Lin Huang Wenjin Ju Gong |
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| Affiliation: | Xu Hanpeng,Gou Lin *,Huang Wenjin,Ju Gong |
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| Abstract: | To determine the expression of morphogen molecule-numb which participated in asymmetric cell division in cultured neural stem cell. Cultured neural stem cell clones which were isolated and expanded in serum-free medium in vitro were subjected to RT-PCR after being passaged several times in vitro and identified by immunochemistry, the amplification production was sequenced and labeled by digoxingemin and in situ hybridization technique was used to detect the cryosection of the neural stem cell clone. Results: most cells(>95%) in the stem cell clone could be detected positive for signal of numb, there was no significant difference among the positive cells in the center and peripheral of the stem cell clone. |
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| Keywords: | numb notch receptor RT-PCR cell culture neural stem cell in situ hybridization mouse |
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