| 阿托伐他汀对脂多糖诱导的THP-1巨噬细胞炎症因子分泌的影响及机制 |
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| 引用本文： | 喻思扬,曾高峰,刘洋,徐健强,曾梦雅,唐业华,曾志英,石小桥,陈莹,王燕,赵国军.阿托伐他汀对脂多糖诱导的THP-1巨噬细胞炎症因子分泌的影响及机制[J].中国动脉硬化杂志,2016,24(7):663-667. |
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| 作者姓名： | 喻思扬 曾高峰 刘洋 徐健强 曾梦雅 唐业华 曾志英 石小桥 陈莹 王燕 赵国军 |
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| 作者单位： | 心血管内科,;心血管内科,;心血管内科,;心血管内科,;心血管内科,;衡阳市中心医院,湖南省衡阳市 421001;麻醉科,湖南省衡阳市 421001;麻醉科,湖南省衡阳市 421001;麻醉科,湖南省衡阳市 421001;麻醉科,湖南省衡阳市 421001;桂林医学院组胚教研室,广西桂林市 541004 |
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| 基金项目： | 国家自然科学基金资助项目(81200218)；湖南省自然科学基金课题(14JJ5016) |
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| 摘 要： | 目的观察阿托伐他汀对脂多糖(LPS)诱导的THP-1巨噬细胞炎症因子分泌的影响,并探讨其机制。方法 100 nmol/L佛波酯孵育THP-1细胞24 h,使其分化为巨噬细胞后,换无血清培养基,加入LPS和(或)阿托伐他汀进行处理。酶联免疫吸附法检测细胞上清液中白细胞介素1β(IL-1β)和白细胞介素18(IL-18)含量,荧光定量PCR检测细胞核苷酸结合寡聚化结构域样受体蛋白1(NLRP1)炎性体的mRNA表达,Western blot检测细胞NLRP1炎性体的蛋白表达。结果阿托伐他汀可呈浓度、时间依赖性抑制LPS诱导的THP-1巨噬细胞IL-1β和IL-18释放;阿托伐他汀可下调THP-1巨噬细胞NLRP1炎性体mRNA和蛋白的表达。结论阿托伐他汀抑制巨噬细胞炎症因子分泌,其作用机制可能与其下调NLRP1炎性体表达有关。
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| 关 键 词： | 阿托伐他汀 抗炎效应 核苷酸结合寡聚化结构域样受体蛋白1炎性体 动脉粥样硬化 |
| 收稿时间： | 2015/8/29 0:00:00 |
| 修稿时间： | 2015/10/21 0:00:00 |
The Effect and Mechanism of Atorvastatin on the THP-1 Macrophage Proinflammatory Cytokines Secretion Induced by Lipopolysaccharide |
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| YU Si-Yang,ZENG Gao-Feng,LIU Yang,XU Jian-Qiang,ZENG Meng-Y,TANG Ye-Hu,ZENG Zhi-Ying,SHI Xiao-Qiao,CHEN Ying,WANG Yan and ZHAO Guo-Jun.The Effect and Mechanism of Atorvastatin on the THP-1 Macrophage Proinflammatory Cytokines Secretion Induced by Lipopolysaccharide[J].Chinese Journal of Arteriosclerosis,2016,24(7):663-667. |
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| Authors: | YU Si-Yang ZENG Gao-Feng LIU Yang XU Jian-Qiang ZENG Meng-Y TANG Ye-Hu ZENG Zhi-Ying SHI Xiao-Qiao CHEN Ying WANG Yan and ZHAO Guo-Jun |
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| Institution: | Department of Cardiovascular Medicine,;Department of Cardiovascular Medicine,;Department of Cardiovascular Medicine,;Department of Cardiovascular Medicine,;Department of Cardiovascular Medicine,;The Central Hospital of Hengyang, Hengyang, Hunan 421001, China;Department of Anesthesiology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China;Department of Anesthesiology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China;Department of Anesthesiology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China;Department of Anesthesiology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China;Department of Histology and Embryology, Guilin Medical University, Guilin, Guangxi 541004, China |
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| Abstract: | |
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| Keywords: | Atorvastatin Anti-inflammatory Effect Nucleotide-binding Oligomerization Domain-like Receptor Protein 1 Inflammasome Atherosclerosis |
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