当归挥发油对高血脂模型大鼠的降血脂作用及血管内皮保护作用

目的观察当归挥发油对高血脂模型大鼠的降血脂作用及血管内皮保护作用。方法 SD大鼠72只,随机分为正常对照组12只和造模组60只,造模组大鼠高脂饲料喂养并灌胃丙基硫氧嘧啶溶液(50 mg/kg),连续4周,造模成功后随机分为模型对照组、氟伐他汀组(0.5 mg/kg)、当归挥发油(ASVO)高、中、低剂量组(60、30、15mg/kg),每组各12只,连续灌胃给药3周,末次给药60 min后股动脉取血,分离血清,测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)含量,计算动脉粥样硬化指数(AI);分离胸主动脉,10%甲醛固定,石蜡包埋,切片,HE染色观察大鼠胸主动脉内皮形态改变,ELISA法测定大鼠血浆内皮素1(ET-1)、血清一氧化氮(NO)和血浆血管性假血友病因子(v WF)的含量。结果模型大鼠血清TC、LDLC水平及AI显著升高(P0.05或P0.01),当归挥发油高、中剂量能够降低高血脂大鼠血清TC、LDLC水平及AI(P0.05);模型大鼠血浆ET-1和血清v WF水平显著升高、血清NO水平显著下降(P0.01),当归挥发油高、中剂量均能降低血浆ET-1和血清v WF水平(P0.05),升高血清NO水平(P0.01)。光镜下可见模型大鼠胸主动脉内皮结构改变明显,而当归挥发油高、中剂量组病变减轻。结论当归挥发油对高血脂模型大鼠有一定的降脂作用,并能有效改善血管内皮结构的损伤。

Protective Effects of Angelica Sinensis Volatile Oil on Levels of Blood Fats and Vascular Endothelial Structure in Hyperlipidemia Rats

Aim To observe the protective effects of Angelica sinensis volatile oil (ASVO) on levels of blood lipids and vascular endothelial structure in hyperlipidemia rats. Methods 72 SD rats were randomly assigned to normal control group of 12 and model group of 60. Rats in the model group were fed with high fat diet and 50 mg/kg propylthiouracil by intragastric administration for 4 weeks. The model rats were randomly divided into the model control group, fluvastatin group (0.5 mg/kg), and high-, medium- and low-dosage of ASVO groups (0,0, 15 mg/kg) with 12 rats in each group. After 3 weeks, artery blood was collected and the content of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC) levels and calculated atherosclerosis index (AI) were measured, the contents of endothelin-1(ET-1) in plasma, nitric oxide(NO) in serum and von willebrand factor(vWF) in plasma were measured with enzyme linked immunosorbent assay (ELISA). Thoracic aorta was separated, 10% formalin-fixed and parrffin-embedded, cut into slice, HE stained and the vascular endothelial structure was observed under light endothelial structure. Results Compared with control group, the content of TC, TG and LDLC in plasma significantly increased (P<0.05), the content of ET-1 and vWF in plasma significantly increased, the content of NO in the serum significantly decreased in hyperlipidemia model group (P<0.05). The content of TC and LDLC in plasma significantly decreased (P<0.05), the content of ET-1 and vWF in plasma significantly decreased, the content of NO in the serum significantly increased in the high and medium dosage of ASVO groups (P<0.05), the structure of the vascular endothelial injury were effectively improved in the high and medium dosage of ASVO groups. Conclusion The certain therapeutic effects of Angelica sinensis volatile oil were determinated in hyperlipidemia rats, and the structure of the vascular endothelial injury was improved.