当归对自发性高血压大鼠心肌miR-122的影响及其生物信息学分析

目的分析当归对自发性高血压大鼠心肌miR-122的影响及其生物信息学分析。方法将自发性高血压大鼠分为当归组、模型组、卡托普利组、当归+卡托普利组,同周龄的Wistar大鼠作为正常对照组,测定用药前后不同组别鼠尾动脉收缩压。分组给药4周后,取大鼠心肌组织进行miRNA表达谱的测定。结果当归可降低自发性高血压大鼠的血压水平。当归组表达上调的miRNA有13个,表达下调的miRNA有16个;卡托普利组表达上调的miRNA有15个,表达下调的miRNA有13个;当归+卡托普利组表达上调的miRNA有4个,表达下调的miRNA有21个。对在三组中都表达上调的miR-122进行生物学过程富集分析,发现与氨基酸转运相关的基因Slc7a1。通过对差异表达的miRNA进行靶基因预测,当归组有8个miRNA含有靶基因Slc7a1,卡托普利组有11个miRNA含有靶基因Slc7a1,当归+卡托普利组有6个miRNA含有靶基因Slc7a1。结论当归对自发性高血压大鼠miR-122表达产生影响,可能通过其靶基因Slc7a1影响内皮功能,进而调节血压,为进一步的研究提供了依据。

Effect of Angelica on miR-122 in Myocardial Tissue of Spontaneously Hypertensive Rats and Its Bioinformatics Analysis

Aim To study the effect of angelica on miR-122 in myocardial tissue of spontaneously hypertensive rats (SHR) and its bioinformatics analysis. Methods All the spontaneously hypertensive rats were divided into angelica group, model group, captopril group and angelica captopril group, the same-age Wistar rats as normal control group, then the systolic blood pressure of the tails of all rats in different groups were measured before and after treatments. After 4 weeks, myocardial tissue of the rats were extracted to test miRNA expression profiling. Results Angelica can reduce blood pressure levels in SHR. 13 miRNAs were found up-regulated and 16 miRNAs down-regulated in angelica group, and 15 miRNAs were found up-regulated and 13 miRNAs down-regulated in captopril group and 4 miRNAs were found up-regulated and 21 miRNAs down-regulated in angelica captopril group. We made a analysis of biological process of miR-122, which was up-regulated in all of the three groups, and an amino acid transport gene Slc7a1 was found. By miRNA target precdiction, 8 miRNAs were found targeted by Slc7a1 in angelica group and 11 miRNAs were found targeted by Slc7a1 in captopril group and 11 miRNAs were found targeted by Slc7a1 in captopril group. Conclusions Angelica can regulate the expression of miR-122, with the mechanism that angelica influenced endothelial function by the target gene Slc7a1 of miR-122 and resulted in regulation of blood pressure, which established a foundation of further research.