口腔粘膜下纤维性变患者外周血淋巴细胞姐妹染色单体交换率研究

采用 Brd U- Giemsa染色法检测了湖南湘潭地区居民中有咀嚼槟榔习惯的口腔粘膜下纤维性变 (OSF)患者2 7人 ,有咀嚼槟榔习惯但未患 OSF的健康者 14人 ,无咀嚼槟榔习惯的正常人 44名的外周血淋巴细胞姐妹染色单体交换率 (SCE率 )。结果显示 :有咀嚼槟榔习惯者 SCE频率显著高于无咀嚼槟榔习惯者 (P<0 .0 0 1) ;患 OSF者SCE频率显著高于健康者 (P<0 .0 0 1)。此研究提示了槟榔咀嚼物中含有某些致突变或恶变成份 ,咀嚼槟榔习惯可严重影响染色体的稳定性 ;OSF为一种癌前状态 ,其发生可能具有一定的遗传背景或易感性

A Study on the Frequency of Sister Chromatid Exchanges in Patients; with Oral Submucous Fibrosis in Peripheral Blood Lymphocytes

The frequency of sister chromatid exchanges (SCEs) in peripheral blood lymphocytes in the patients (n=27) with oral submucous fibrosis (OSF) with the habit of chewing areca nuts were determined by BrdU Giemsa staining. The healthy persons (n=14) with the same habit and the normal controls without the habit (n=44) were studied at the same time. The results showed that the frequency of SCEs in the first two groups were obviously higher than that in the last one (P<0.001). The frequency of SCEs in the patients were also higher than that of the healthy persons with the habit (P<0.001). These suggest that there are some substances in the areca nuts which can induce the mutation and/or malignant transformation of cells. The habit of chewing areca nuts might seriously disturbe the stability of chromosome. OSF is a precancer condition. The occurance of it perhaps has some hereditary background or genetic susceptibility.;