4-苯乙烯磺酸马来酸酐共聚物抑制HSV-2感染研究

目的:探索4-苯乙烯磺酸马来酸酐共聚物(PSM)对单纯疱疹病毒Ⅱ型(HSV-2)感染细胞或小鼠的抑制作用。方法:建立Vero-ICP10细胞系,通过Luciferase检测不同浓度PSM对感染细胞中HSV-2的抑制作用,并通过In cell western技术检测HSV-2 gD蛋白表达,确定不同PSM浓度对HSV-2 gD蛋白表达的抑制作用;建立HSV-2阴道感染小鼠模型,观察PSM对HSV-2经生殖道感染小鼠的抑制作用。结果:Luciferase实验和In cell western实验结果均表明PSM具有抗HSV-2感染细胞的作用;小鼠模型中,5%的PSM能有效防止小鼠感染HSV-2(P<0.001)。结论:PSM在体内和体外模型中均可以抑制HSV-2感染。

Antiviral effect of PSM against herpes simplex virus type 2

Objective: To investigate the antiviral effect of polymer of styrenesulfonic acid and maleic acid(4-styrenesulfonic acid-co-maleic acid)(PSM) against herpes simplex virus type 2(HSV-2).Methods: A Vero-ICP10 cell line containing virus-controlled Luciferase as indicator was established for analyzing the effect of different concentrations of PSM on HSV-2 infection.An In cell western assay was also used for detecting intracellular viral protein expression.PSM inhibitory activity was further evaluated in a HSV-2 vaginal challenge murine model.Results: The results of in vitro analyses showed that PSM could protect cell from HSV-2 infection,and the murine model showed that 5% PSM protected 95% of the animals from HSV-2 infection(P<0.001).Conclusion: PSM is a potential agent for the prevention of HSV-2 infection through sexual route.