脱镁叶绿环类衍生物的分离,制备及鉴定

自蚕砂糊状叶绿素分得的叶绿素 a(1),于不同条件下经酸降解、脱羧焦化、甲酯化、氧化,在各步中可得脱镁叶绿素 a(2)、脱镁叶绿酸 a(3),焦脱镁叶绿酸 a(4)、甲基脱镁叶绿酸 a(5)、甲基焦脱镁叶绿酸a(6)、10-羟基甲基脱镁叶绿酸 a(7)和红紫素7-内酯二甲酯(8)。7和8的 ~1HNMR 和 MS 数据为首次报道。

SEPARATION,PREPARATION AND IDENTIFICATION OF PHORBIN DERIVATIVES

Seven phorbin derivatives and one purpurin derivative were prepared from crude chlorophyll in silkworm excrement by selective hydrolysis and allomerization.It was shown by the spectra of ~1HNMR,MS,IR and UV that theyare chlorophyll a(1),pheophytin a(2),pheophorbide a(3),pyropheophorbide a(4),methylpheophorbide a(5),methylpyropheophorbide a(6),10-hydroxymethyl-pheophorbide a(7) and purpurin 7 lactone-dimethylester(8).