CD44在大鼠肾移植急性排斥反应中的表达

目的:探讨移植肾组织CD44的表达及血清中可溶性CD44的含量与急性排斥反应的关系。方法:雄性Wistar大鼠和SD大鼠分别作为供体和受体,共分为四组,采用改进的Blom法大鼠原位肾移植模型。免疫组织化学染色法检测移植肾组织CD44分子的表达;酶联免疫吸附试验测定术后血清中可溶性CD44水平的变化。结果:移植肾组织CD44分子的表达在同种异体移植组显著高于同品系移植组、手术对照组及药物治疗组(均P<0.05);移植肾组织CD44分子的表达与急性排斥反应呈正相关(皮质:r=0.734,髓质:r=0.670,均P<0.01);发生急性排斥反应的移植肾组织CD44分子的表达与Banff急性排斥反应指数无相关性(P>0.05);血清中可溶性CD44分子各组间差异无统计学意义,与急性排斥反应及Banff指数均无相关性(均P>0.05)。结论:CD44分子在肾移植急性排斥反应的发病机制中起着重要作用,为进一步提高移植排斥反应防治水平提供理论依据。

Expression of CD44 in rat renal allografts during acute rejection

Objective:To assess relationship between the renal graft expression of CD44, serum soluble CD44 and the graft acute rejection.Methods:Male wistar and Sprague-Dawley rats were used as donors and recipients, respectively. Rat orthotopic kidney transplantation was performed according to a modification of the method decribed by Blom. Experimental rats were divided into 4 groups. Immunohistochemical staining was used to investigate the expression of CD44 on untransplanted kidneys and grafts. The levels of serum soluble CD44 was sequentially monitored by means of the enzyme-linked immunosorbent assay method (ELISA) at 5 or 7 days post-transplantation.Results:CD44 expression increased significantly in the acute rejection group compared with the non-rejection groups (P< 0.05). Renal grafts CD44 expression correlated with acute rejection (cortex: r= 0.744, P< 0.01; medulla: r= 0.811, P< 0.01), whereas in rats developing acute rejection it did not correlate with Banff scores (P> 0.05). Serum soluble CD44 showed no difference between the acute rejection group and the non-rejection groups (P> 0.05). There was no correlation between serum soluble CD44 and acute rejection (P> 0.05) or acute rejection Banff scores (P> 0.05).Conclusions:CD44 plays an important role in the pathogenesis of acut allograft rejection. Further studies of CD44 may help to promote the development of new approaches for controlling and preventing graft acute rejection.