Expression of NF-κB in Schwann cells and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in rats |
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引用本文: | 王永堂,鲁秀敏,余瑛,杨艳红,高洁.Expression of NF-κB in Schwann cells and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in rats[J].中国人民解放军军医大学学报,2007(2). |
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作者姓名: | 王永堂 鲁秀敏 余瑛 杨艳红 高洁 |
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作者单位: | State Key Laboratory of Trauma Burns and Combined Injury Institute of Surgery Research,Daping Hospital,Third Military Medical University,College of Bioengineering Chongqing Institute of Technology,College of Bioengineering Chongqing Institute of Technology,College of Bioengineering Chongqing Institute of Technology,State Key Laboratory of Trauma Burns and Combined Injury,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China,Chongqing 400050,China,Chongqing 400050,China,Chongqing 400050,China,Chongqing 400042,China |
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基金项目: | Supported by the National Natural Science Foundation of China (No. 30600665),the Opening Project Foundation of State Key Laboratory of Trauma. Burns and Combined Injury (No. 2006A-3),the Youth Scientific Research Foundation of Third Military Medical University (No.06XG048) |
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摘 要: | Objective: To explore the expression of nuclear factor-kappa B (NF-κB) in Schwann cells (SCs) and its effect on motor neuron apoptosis in spinal cord following sciatic nerves injury in adult rats. Methods: Thirty-six adult Sprague-Dawley (SD) rats were divided randomly into normal control group (n=6), and sciatic nerves crushing group (n=30). and the later was further equally randomized into 5 subgroups: 1. 3. 7. 11. and 21 d post-injury groups. The expression of NF-κB of normal and injured nerves were examined by immunohistochemistry staining, and the apoptosis of motor neurons in spinal cord of lumbar 4 to lumbar 6 (L4-L6) was investigated by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNED assay. Both were quantitated by image analysis. Results: In crushing group, except 21 d post-injury group, the expression of NF-κB was markedly higher than that in the normal control group (P<0. 05,P<0. 01). At 1 d after sciatic nerves crushing, the expression of NF-κB was obviously up-regulated, reached peak at 3 d. and recovered at 21 d. The same trend was observed in the time-course on motor neuron apoptosis after sciatic nerves injury. Correlation analyses revealed that motor neuron apoptosis was significantly and positively correlated with the expression of NF-κB following sciatic nerves injury (r=0. 976 0,P<0. 01). Conclusion: After injury of sciatic nerves, the presence and up-regulation of NF-κB in SCs may be involved in motor neuron apoptosis in L4-L6 spinal cord.
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