Listeria monocytogenes is sensed by the NLRP3 and AIM2 inflammasome |
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| Authors: | Sarah Kim Franz Bauernfeind Andrea Ablasser Gunther Hartmann Katherine A. Fitzgerald Eicke Latz Veit Hornung |
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| Affiliation: | 1. Institute for Clinical Chemistry and Pharmacology, Unit for Clinical Biochemistry, University Hospital, University of Bonn, Bonn, Germany;2. Division of Infectious Diseases & Immunology, University of Massachusetts Medical School, Worcester, MA, USA;3. Institute of Innate Immunology, University Hospital, University of Bonn, Bonn, Germany |
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| Abstract: | The inflammasome pathway functions to regulate caspase‐1 activation in response to a broad range of stimuli. Caspase‐1 activation is required for the maturation of the pivotal pro‐inflammatory cytokines of the pro‐IL‐1β family. In addition, caspase‐1 activation leads to a certain type of cell death known as pyroptosis. Activation of the inflammasome has been shown to play a critical role in the recognition and containment of various microbial pathogens, including the intracellularly replicating Listeria monocytogenes; however, the inflammasome pathways activated during L. monocytogenes infection are only poorly defined. Here, we demonstrate that L. monocytogenes activates both the NLRP3 and the AIM2 inflammasome, with a predominant involvement of the AIM2 inflammasome. In addition, L. monocytogenes‐triggered cell death was diminished in the absence of both AIM2 and NLRP3, and is concomitant with increased intracellular replication of L. monocytogenes. Altogether, these data establish a role for DNA sensing through the AIM2 inflammasome in the detection of intracellularly replicating bacteria. |
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| Keywords: | AIM2 Caspase‐1 Inflammasome Listeria monocytogenes NLRP3 |
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