Neuron‐specific expression of atp6v0c2 in zebrafish CNS |
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| Authors: | Ah‐Young Chung Myoung‐Jin Kim Dohyun Kim Sangsu Bang Sun Wook Hwang Chae Seung Lim Sanggyu Lee Hae‐Chul Park Tae‐Lin Huh |
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| Affiliation: | 1. School of Life Science and Biotechnology, Kyungpook National University, Daegu, Korea;2. Graduate School of Medicine, Korea University, Ansan, Gyeonggido, Korea;3. Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea |
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| Abstract: | Vacuolar ATPase (V‐ATPase) is a multi‐subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V0 domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron‐specific expression of atp6v0c2, a novel isoform of the V‐ATPase c‐subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome‐wide analysis of the zebrafish mibta52b mutant designed to identify genes differentially regulated by Notch signaling. Whole‐mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post‐mitotic neurons. The ATP6V0C2 protein is co‐localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss‐of‐function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability. Developmental Dynamics 239:2501–2508, 2010. © 2010 Wiley‐Liss, Inc. |
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| Keywords: | V‐ATPase neuron Notch signaling zebrafish |
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