IL‐10 produced by activated human B cells regulates CD4+ T‐cell activation in vitro |
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| Authors: | Jean‐David Bouaziz Maud Maho‐Vaillant Anne Saussine Martine Bagot Philippe Musette |
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| Affiliation: | 1. INSERM U976, Saint Louis Hospital, Skin Research Center, Paris, France;2. University of Paris 7, Saint Louis Hospital, Paris, FranceThese authors have contributed equally to this work.;3. INSERM U905, Charles Nicolle Hospital, Rouen, France;4. University of Rouen, Charles Nicolle Hospital, Rouen, France;5. University of Paris 7, Saint Louis Hospital, Paris, France |
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| Abstract: | IL‐10‐producing regulatory B cells have been identified in mice and shown to downregulate inflammation, making them potentially important for maintenance of tolerance. In this study, we isolated B cells from human blood and spleen, and showed that after a short period of ex vivo stimulation a number of these cells produced IL‐10. The IL‐10‐producing B cells did not fall within a single clearly defined subpopulation, but were enriched in both the memory (CD27+) and the transitional (CD38high) B‐cell compartments. Combined CpG‐B+anti‐Ig stimulation was the most potent IL‐10 stimulus tested. B cells stimulated in this way inhibited CD4+CD25? T‐cell proliferation in vitro by a partially IL‐10‐dependent mechanism. These findings imply that manipulating IL‐10 production by human B cells could be a useful therapeutic strategy for modulating immune responses in humans. |
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| Keywords: | B cells IL 10 Tolerance |
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