杜仲对大鼠骨髓间充质干细胞增殖的影响

【目的】探讨杜仲对大鼠骨髓间充质干细胞(BMSCs)增殖的影响。【方法】分别取10日龄SD大鼠胫骨、股骨的骨髓,过滤离心后将所得BMSCs进行体外单层培养、扩增,采用DMEM培养基,37℃、体积分数5%CO2条件培养。在细胞达到80%～90%融合时,使用2.5 g/L胰酶消化传代。将传代细胞分别置于空白血清培养液(对照组)、杜仲水提取物含药血清及杜仲醇提取物含药血清培养液进行培养,观察细胞在3种条件下的形态、贴壁生长、增殖、集落等情况,采用四甲基偶氮唑盐(MTT)法绘制生长曲线,比较3种培养条件下达到100%融合的时间。【结果】杜仲水提取物组、杜仲醇提取物组对大鼠BMSCs增殖的影响显著高于空白血清对照组(P<0.05),且杜仲醇提取物组作用优于杜仲水提取物组(P<0.05)。【结论】杜仲对大鼠BMSCs增殖具有一定促进作用。

Effect of Cortex Eucommiae on Proliferation of Bone Marrow Stromal Cells in Rats

Objective To investigate the effect of Cortex Eucommiae on the proliferation of bone marrow stromal cells(BMSCs) in rats.Methods Ten-month-old SD rats were used as the experimental animals.The bone marrow in rats tibia and femur was taken out for the preparation of BMSCs after filtration and centrifugalization.BMSCs were cultured in DMEM culture medium at 37℃ with 5% CO2.When cell fusion occurred in 80%～90% of BMSCs,BMSCs were digested and subcultured with 2.5 g/L pancreatin.The subcultured BMSCs were cultured with blank serum,serum containing water-extract of Cortex Eucommiae,serum containing alcohol-extract of Cortex Eucommiae,respectively.After culture,the morphological features,adherence growth,proliferation and colony of BMSCs were observed,the growth curve was drawn according to the results of MTT assay,and the time for cell fusion in 100% of BMSCs was compared under different culture conditions.Results Serum containing water-extract of Cortex Eucommiae and serum containing alcohol-extract of Cortex Eucommiae had stronger effect on BMSCs proliferation than blank serum,and the effect of serum containing alcohol-extract of Cortex Eucommiae was the strongest(P<0.05).Conclusion Cortex Eucommiae shows certain effect on promoting the proliferation of BMSCs in rats.