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| 高糖诱导氧化应激对胰岛细胞功能的影响 | |
| 引用本文： | 陈莹晖,李裕明,林松挺,谭光宏.高糖诱导氧化应激对胰岛细胞功能的影响[J].海南医学院学报,2012,18(1):12-14. |
| 作者姓名： | 陈莹晖 李裕明 林松挺 谭光宏 |
| 作者单位： | 1. 海南医学院附属医院重症医学科,海南海口,570102 2. 华中科技大学同济医学院附属协和医院内分泌科,湖北 武汉,430022 3. 海南省海口市人民医院,海南海口 57020 4. 海南医学院热带病实验室,海南海口,571101 |
| 基金项目： | 海南医学院科研基金学报项目 |
| 摘 要： | 目的:研究不同浓度葡萄糖对INS-1细胞及胰岛分泌功能的影响,并探讨氧化应激在葡萄糖对INS-1细胞功能损伤中的作用。方法:将不同浓度葡萄糖(11.1、16.7、22.2、33.3mmol/L)作用于INS-1细胞,镜下观察INS-1细胞的形态改变,应用可见分光光度计测定丙二醛(MDA)含量,运用ELISA方法检测葡萄糖刺激的胰岛素分泌(GSIS)。结果:随着葡萄糖浓度的增加,INS-1细胞的凋亡增多,MDA含量逐渐增加,GSIS值逐渐下降,不同浓度葡萄糖组间MDA含量、GSIS值差异有统计学意义(P均<0.01)。结论:高糖可促进INS-1细胞凋亡,在高糖作用下活性氧物质(ROS)代谢产物MDA增多,胰岛细胞胰岛素分泌功能受损。 |
| 关 键 词： | 氧化应激 胰岛β细胞 胰岛功能 |
Islet dysfunction induced by oxidative stress under high glucose condition |
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| CHEN Ying-hui , LI Yu-ming , LIN Song-ting , TANG Guang-hong.Islet dysfunction induced by oxidative stress under high glucose condition[J].Journal of Hainan Medical College,2012,18(1):12-14. | |
| Authors: | CHEN Ying-hui LI Yu-ming LIN Song-ting TANG Guang-hong |
| Institution: | CHEN Ying-hui,LI Yu-ming,LIN Song-ting,TANG Guang-hong (1.ICU,The Affiliated Hospital of Hainan Medical College,Haikou 570102,Chiana;2.Department of Endocrinology,Affiliated Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;3.Haikou Municipal Hospital,Haikou 570208,Chiana;4.Tropical Diseases Laboratory,Hainan Medical College,Haikou 571101,China.) |
| Abstract: | Objective: To determine the dose-dependent effect of glucose on the secretary activity of INS-1 cells,and to explore the role of oxidative stress in glucose-meidated functional damage of INS-1 cells.Methods: INS-1 cells were treated with glucose at concentrations of 11.1、16.7、22.2、33.3 mmol/L,then the morphological changes were observed under a light microscope.Malondialdehyde(MDA) content in the culture media was determined by spectrophotometry and the glusoe-stimulated insulin secretion(GSIS) was assessed by ELISA.Results:As the glucose dose increased,the INS-1 cell apoptosis increased,MDA concentration gradually elevated while the GSIS gradually decreased showing significant differences among groups treated with different concentration of glucose.Conclusions:Under high glucose conditions,the reactive oxygen species(ROS) metabolite MDA increases,leading to apoptosis and thereby impaired insulin secretion in isle |
| Keywords: | Oxidative stress Islet β-cells Islet function |
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