| 高增殖潜能内皮祖细胞促进脐血造血祖细胞体外扩增 |
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| 引用本文： | 段华新,刘畅,杨新宇,毛平,徐艳丽,罗畅如,王彩霞,谢健晋,张玉平.高增殖潜能内皮祖细胞促进脐血造血祖细胞体外扩增[J].广州医学院学报,2012,40(1):1-4. |
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| 作者姓名： | 段华新 刘畅 杨新宇 毛平 徐艳丽 罗畅如 王彩霞 谢健晋 张玉平 |
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| 作者单位： | 1. 湖南师范大学第一附属医院 湖南省人民医院血液肿瘤科,湖南长沙,410006
2. 广州市第一人民医院血液科,广东广州,510180 |
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| 基金项目： | 中国博士后科学基金，湖南省自然科学基金，长沙市科技计划，湖南省教育厅科学研究基金，湖南省人民医院仁术基金 |
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| 摘 要： | 目的：研究脐血来源高增殖潜能内皮祖细胞支持脐血造血干／祖细胞体外扩增的能力。方法：利用第3代的HPP—EPC联合细胞因子培养脐血造血干／祖细胞，分因子组、非接触培养组和接触培养组。体外培养脐血CD34＋细胞10d后，从细胞总数，CD34＋细胞数，各系造血集落形成细胞数等比较各组的扩增效率。结果：接触组细胞总数扩增倍数（44．6±8．7）倍和非接触组（39．5±7．3）倍均高于因子组（24．8±5．6）倍，P〈0．01。接触组CD34＋细胞数扩增倍数（8．8±2．1）倍高于因子组（2．9±0．6）倍和非接触培养组（3．3±0．6）倍，P〈0．01。接触组集落扩增倍数和非接触组均分别高于因子组，P〈0．05，且接触组各造血集落扩增数均分别高于非接触组，各P〈0．05。接触组扩增后CD34＋CD38＋细胞（10．8％±2．7％）明显高于因子组（4．1％±0．9％）和非接触培养组（3．8％±0．8％），P〈0．01。结论：脐血来源高增殖潜能内皮祖细胞能支持脐血CD34＋细胞体外扩增。
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| 关 键 词： | 高增殖潜能内皮祖细胞 脐血 造血干／祖细胞 扩增 |
High proliferative potential endothelial progenitor cells support the expansion of hematopoietic progenitor cells from human umbilical cord blood |
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| DUAN Hua-xin
,
LIU Chang
,
YANG Xin-yu
,
MAO Ping
,
XU Yan-li
,
LUO Chang-ru
,
WANG Cai-xia
,
XIE Jian-jin
,
ZHANG Yu-ping.High proliferative potential endothelial progenitor cells support the expansion of hematopoietic progenitor cells from human umbilical cord blood[J].Academic Journal of Guangzhou Medical College,2012,40(1):1-4. |
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| Authors: | DUAN Hua-xin LIU Chang YANG Xin-yu MAO Ping XU Yan-li LUO Chang-ru WANG Cai-xia XIE Jian-jin ZHANG Yu-ping |
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| Institution: | 1 Department of Hematology and Oncology, The First Affiliated Hospital of Hunan Normal University/Hunan Pro- vincial People＇ s Hospital, Changsha 410006, China; 2. Department of Hematology, First Municipal People＇ s Hospital, Guangzhou 510180, China) |
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| Abstract: | Objective： To investigate whether human cord blood derived high proliferative potential endothelial progenitor cells （HPP-EPC） can support the expansion of heraatopoietie progenitor cells from human umbilical cord blood. Methods： Passage 3 HPP-EPCs were used to expand CD34 ＋ cells from fresh umbilical cord blood in 3 groups ： cytokines group as control, non contact group and contact group. After 10 d culture, total cell number, CD34 ＋ cell number, colonies of hematopoietic progenitors were counted to analyze the expansion effects in each group. Results： After 10 d expansion, the expansion folds of total eell number in both contact group （44.6 ± 8.7 ） and non contact group （39.5 ± 7.3 ） were higher than in cytokine group （24.8 ± 5.6, both P 〈 0.01 ）. The expansion fold of CD34 ＋ was higher in contact group （ 8.8 ± 2.1 ） than in non contact group （ 3.3 ± 0.6 ） or cytokine group （ 2.9 ± 0.6 ） （ both P 〈 0.01 ）. The expansion folds of colonies in contact group and in non contact group were higher than those in cytokine group （ all P 〈 0.05 ）. And the expansion folds of colony number of hematopoietic progenitors in contact group were higher than those in non contact group （ all P 〈0.05）. The rate of CD34 ＋ CD38- cells after expansion ex vivo in contact group （10.8% ± 2.7% ） was higher than that in cytokine group （4.1%± 0.9% ） and non contact group （3.8% ± 0.8% ）. Conclusion： Human umbilical cord blood derived HPP-EPC can support the expansion of CD34 ＋ hematopoietic stem/progenitor cells from human umbilical cord blood. |
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| Keywords: | high proliferative potential endothelial progenitor cells umbilical cord blood hematopoietic progenitor cells expansion |
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