步长脑心通对脑缺血再灌注大鼠脑组织一氧化氮合酶表达的影响

【目的】探讨步长脑心通对脑缺血的保护作用机制。【方法】选用Wistar大鼠,随机分为正常组、假手术组、模型组与治疗组,再分为12 h和24 h 2个亚组,治疗组给予步长脑心通灌胃(剂量为0.45 g.kg-1.d-1);采用大脑中动脉线栓法复制大鼠脑缺血再灌注模型,应用免疫组织化学SP法检测大鼠脑组织一氧化氮合酶iNOS、eNOS的表达,采用图像分析仪测定光密度值,光镜下分析阳性细胞百分率。【结果】与模型组比较,治疗组iNOS表达显著降低(P<0.05),eNOS表达显著增高(P<0.05)。【结论】步长脑心通能降低脑缺血再灌注大鼠脑组织iNOS表达、增强eNOS表达,从而抑制神经细胞凋亡,发挥脑保护作用。

Influences of Buchang Naoxintong on Expression of Cerebral Nitric Oxide Synthases in Rats with Focal Cerebral Ischemia-reperfusion

Objective To study the neuroprotection mechanism of Buchang Naoxintong for focal cerebral ischemia.Methods Wistar rats were randomized into normal control group,pseudo operation group,model group and treatment group(treated with gastric gavage of Buchang Naoxintong 0.45 g·kg-1·d-1).Each group was divided into 12-h subgroup and 24-h subgroup.The focal cerebral ischemia-reperfusion model was established in rats by using the method of thread inserting into left middle cerebral artery.Expression of cerebral inducible nitric oxide synthase(iNOS) and endothelial nitric oxide synthase(eNOS) was measured by SP immunohistochemistry.The optical density value(OD) was measured by imaging analysis,and the percentage of cells with iNOS and eNOS positive expression was analyzed under light microscope.Results Treatment group had lower expression level of iNOS but higher expression level of eNOS than those in the model groups(P<0.05).Conclusion Buchang Naoxintong can decrease the expression of iNOS and increase the expression of eNOS in cerebral ischemia-reperfusion rats,which may contribute to the protection of neural function by inhibiting apoptosis of neurons.