兔膝关节半月板水平裂损伤修复的实验研究

目的：通过构建动物半月板水平裂损伤模型，观察不同方法对其半月板损伤模型修复的影响，为临床治疗半月板及软骨损伤提供依据。方法：36只成年新西兰白兔行膝关节外侧半月板水平裂损伤后随机分为3组：空白对照组A、纤维蛋白凝胶组B、富血小板血浆凝胶组c，每组12只。3组分剐于术后4、8、12周分3次处死取材，肉眼行大体形态观察，在光学显微镜观察各组半月板损伤区域愈合情况。结果：A组均未愈合，B组的II级愈合率占58．3％，C组的Ⅱ级和Ⅲ级愈合率各占50．0％；A组的愈合效果明显差于B组的愈合效果（P=0．014），也低于C组的愈合效果（P〈0．01），而C组的愈合效果要B组的愈合效果（P〈0．01）。结论：半月板水平裂损伤后不可自然愈合，纤维蛋白凝胶可促进半月板水平裂损伤的愈合但为瘢痕愈合，富血小板血浆凝胶不仅可以促进半月板水平裂损伤愈合，半月板水平裂缺损区为纤维软骨性愈合。

Repair of meniscus horizontal fissure injury of knee joints in rabbits: an experimental study

Objective：To investigate the impact of various approaches on meniscus repair via establishing the animal model of meniscus horizontal fissure injury, thereby offering proofs for repairing meniscus and cartilage injury in clinics. Methods ： A total of 36 adult New Zealand white rabbits were subjected to meniscus horizontal fissure injury of knee joints and were randomly assigned into three groups, namely the blank control group （ group A）, fibrin gel-treated group （group B ） and platelet-rich plasma gel-treated group （group C ）, respectively. Rabbits of each group were sentenced at weeks 4, 8 and 12 after operation. Meniscuses were extracted for gross morphological observation and optical microscopic examination to determine the repair of injured regions. Results： Group A showed no evidence of repair. By contrast, group B presented a rate of 58.3% for grade II repair, and group C was found to have the rate of 50.0% for both grade II and III repair, suggesting a remarkably lower repair rate of group A as compared with that of group B （ P = 0. 014） and group C （ P 〈 0. 01 ） as well as better repair conditions of group C when compared with that of group C （P 〈 0.01 ）. Conclusion： In contrast to lack of spontaneous repair of meniscus horizontal fissure injury, fibrin glue may form scars in spite of the capacity of promoting repair. Whereas platelet-rich plasma gel is not only capable of promoting meniscus repair, but inducing fibrocartilage healing where meniscus horizontal fissure defect is located as well.