HPLC法测定小儿清热止咳口服液中苦杏仁苷、甘草苷和甘草酸的含量

目的 采用高效液相色谱法同时测定小儿清热止咳口服液中的苦杏仁苷、甘草苷和甘草酸. 方法 采用Hypersil C18色谱柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱;苦杏仁苷检测波长:207nm;甘草苷和甘草酸检测波长:237nm;柱温:35℃. HT5"H结果 苦杏仁苷、甘草苷和甘草酸分别在50.82~508.16μg·mL-1、7.43~74.30μg·mL-1、30.68~306.76μg·mL-1浓度范围内线性关系良好(相关系数r均大于0.999);3种成分的方法精密度RSD分别为3.98%、2.36%、1.07%,平均加样回收率分别为97.64%、105.33%、102.26%.结论 该方法操作简单可行、准确灵敏、重复性良好,可用于测定小儿清热止咳口服液中上述3种成分的含量.

Determination of Amygdalin,Licorice glycosides and Glycyrrhizic Acid in Pediatric oral liquid Qingrezhike by HPLC

OBJECTIVE To establish the method for simultaneou s determinatio n of amygdalin,licorice glycosides and glycyrrhizic acid in Pediatric oral Qing rezhike by HPLC.METHODS The Hypersil C18column maintained at 35℃ with a gradient mobile phase system consisting of AC N-0.1% phosphoric acid solution was employed for sample analysis.The wavelength of the UV detector was set at 207nm for a mygdalin determination,whilst 237nm for liquiritin and glycyrrhizic acid eter mination.RESULTS The linear ranges of amygdalin,liquiritin and glycyrrhizi c acid were 50.82~508.16μg·mL-1、7.43~74.30μg·mL-1、30.68~306.76μg·mL-1,resp ectively(r>0.999).The precisions of the method was 3.98%,2.36%,1.07%,the avera ge recoveries was 97.64%,105.33%,102.26% respectively.CONCLUSION WTBZ The method is convenient with a good reproducibility and could determine3 active in gredients of Pediatric oral Qingrezhike,which could be used as a quantitative analysis for preparations.