| 离子色谱法测定盐酸小檗碱片的含量 |
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| 引用本文： | 王莹,刘继华,罗瑢,范亚刚.离子色谱法测定盐酸小檗碱片的含量[J].海峡药学,2017(3). |
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| 作者姓名： | 王莹 刘继华 罗瑢 范亚刚 |
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| 作者单位： | 云南省食品药品检验所 昆明 650011 |
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| 摘 要： | 目的 建立离子色谱法测定盐酸小檗碱盐含量的方法.方法 色谱条件为:IonPacTM AG19(4×50mm)为保护柱;IonPacTM AG19(4×250mm)为分析柱;25.00mmol·L-1 KOH溶液为淋洗液以流速1.00mL·min-1等度洗脱;柱温:30.0℃;检测方式:电导检测;抑制电流:75mA;进样体积:25μL.HT5"H结果 盐酸盐在2.0483~40.9663μg·mL-1呈良好线性关系(r2=1.0000);精密度RSD%=0.10%,平均回收率为101.45%(RSD%=1.80%);.结论HT6K 本法操作简便、快速,结果准确,重复性好,灵敏度高,可用于控制盐酸小檗碱的含量.
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| 关 键 词： | 离子色谱 盐酸小檗碱片 含量测定 |
Ion Chromatography Determination of the Content of Berberine Hydrochloride Tablets |
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| WANG Ying,LIU Ji-hua,LUO Rong,FAN Ya-gang.Ion Chromatography Determination of the Content of Berberine Hydrochloride Tablets[J].Strait Pharmaceutical Journal,2017(3). |
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| Authors: | WANG Ying LIU Ji-hua LUO Rong FAN Ya-gang |
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| Abstract: | OBJECTIVE A method for the determination of berberine hydrochlo ride tablets by ion chromatography was established.METHODS Ion chromatography was performed on IonPac AG19(4×250mm)anion exchange chromatogra phy column with aguard column of IonPacTM AG19(4×50mm);25.00mmol·L-1 KOH was used as the isocratic eluent,at a flow rate of 1.00mL·min-1 with suppressed conductivity detection.RESULTS The linear range of determination was 2.0483~40.9663μg·mL-1(r2=1.0000).The r ecovery rate was 101.45%(RSD%=1.80%),and the degree of precision was 0.10%.CONCLUSION The method is simple,rapid,accurate,reproducible,high sensitivity,can be used to determine the content in berberine hydrochlor ide tablets. |
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| Keywords: | Ion chromatography Berberine hydrochloride tablets Content determination |
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